Z-DEVD-FMK

Catalog No.S7312 Batch:S731204

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Technical Data

Formula

C30H41FN4O12
Molecular Weight 668.66 CAS No. 210344-95-9
Solubility (25°C)* In vitro DMSO 100 mg/mL (149.55 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
2%DMSO 30%PEG400 5%Tween80 63%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 1.000mg/ml (1.50mM) Taking the 1 mL working solution as an example, add 20 μL of 50 mg/ml clarified DMSO stock solution to 300 μL of PEG 400, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 630 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Z-DEVD-FMK (Caspase-3 Inhibitor) is a specific, irreversible Caspase-3 inhibitor, and also shows potent inhibition on caspase-6, caspase-7, caspase-8, and caspase-10.
Targets
Caspase-3 [1]
In vitro Z-DEVD-FMK (1–200 μM) inhibits D4-GDI cleavage and apoptosis in a concentration-dependent manner. This compound reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. It (100μM) attenuates OxyHb-induced cell detachment, reduced caspase-2 and -3 activities, abolishes OxyHb-induced DNA ladders, and prevents OxyHb-induced cleavage of PARP in cultured brain microvessel endothelial cells. This chemical (100 μM) blocks MPP+-induced increases in caspase-3 enzyme activity. It dose dependently blocks 6-OHDA-induced apoptotic cell death with IC50 of 18 μM.
In vivo Z-DEVD-FMK, before and after injury, markedly reduces post-traumatic apoptosis, and significantly improved neurological recovery.

Protocol (from reference)

Kinase Assay:[5]
  • Caspase activity assay

    Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade
Cell Assay:[5]
  • Cell lines

    N27 cells

  • Concentrations

    ~50 μM

  • Incubation Time

    24 hours

  • Method

    N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.
Animal Study:[2]
  • Animal Models

    Male Sprague Dawley rats with Brain trauma.

  • Dosages

    160 ng

  • Administration

    Intracerebroventricular administration

References

  • https://pubmed.ncbi.nlm.nih.gov/9485209/
  • https://pubmed.ncbi.nlm.nih.gov/9295387/
  • https://pubmed.ncbi.nlm.nih.gov/10997751/
  • https://pubmed.ncbi.nlm.nih.gov/11157197/
  • https://pubmed.ncbi.nlm.nih.gov/17045926/

Customer Product Validation

<p>(J,K)After pre-treated with Caspase-3 inhibitor Z-DEVD-FMK 50μM, the apoptotic rates of cells induced by E Platinum in BGC-823, MGC-803, and SGC-7901 cells were detected by Annexin V/PI double-staining assay. Ann.V-/PI- as healthy cells, Ann.+/PI- as early apoptotic cells, Ann.+/PI+ probably as late apoptotic cells. Data were shown as means SD for three independent experiments (*P<0.05 and **P<0.01 compared with control, ##P<0.01 compared to Z-DEVD-FMK+40 μM E Platinum group with treatment of 40 μM E Platinum).</p>

, , Mol Carcinog, 2016, 56(1):218-231

Immunoblotting analysis was performed for the active-caspase-3 and cleavage of MCL-1 in MM.1S cells treated with z-DEVD-fmk (20 μM, 1 h) and following GSK126 (25 μM, 24 h).

Data from [ , , Oncotarget, 2017, 8(2):3396-3411 ]

Caspase activation degrades Beclin-1. (A and B) After pretreatment with z-VAD-fmk (20 μM) or z-DEVD-fmk (20 μM) for 1 h, K562 cells were exposed to BIIB021 (400 nM) while 32Dp210-T315I cells were exposed to BIIB021 (200 nM) for 24 h. Whole-cell lysates were subjected to western blot analysis to examine the expression of Beclin-1, LC3I/II, p62, PARP and caspase-3.

Data from [ , , INTERNATIONAL JOURNAL OF ONCOLOGY, 2016, 48:1710-1720. ]

Selleck's Z-DEVD-FMK Has Been Cited by 113 Publications

Severe fever with thrombocytopenia syndrome virus infection of the male reproductive tract induces pathology and inflammation [ Nat Microbiol, 2026, 11(5):1286-1301] PubMed: 41882300
Epidermal MHC-II-mediated NK cell recruitment triggers keratinocyte pyroptosis, facilitating pathogenesis of psoriasis [ Exp Mol Med, 2026, 58(5):1573-1588] PubMed: 42104015
FOXO3 upregulates and activates GSDME to trigger myeloma cell pyroptosis [ Int J Biol Sci, 2026, 22(4):1793-1806] PubMed: 41694578
ADA2-deficient cells exhibit increased levels of cell death and metabolic disturbances [ Cell Death Discov, 2026, 12(1)167] PubMed: 41866403
Cauloside A interacts with TLR4 to induce JNK/Caspase-3/GSDME-dependent pyroptosis in non-small cell lung cancer cells [ Int Immunopharmacol, 2026, 181:116687] PubMed: 42085840
Neutrophil extracellular traps license macrophage production of chemokines to facilitate CD8+ T cell infiltration in obstruction-induced renal fibrosis [ Protein Cell, 2025, pwaf020] PubMed: 39998389
Portimine A toxin causes skin inflammation through ZAKα-dependent NLRP1 inflammasome activation [ EMBO Mol Med, 2025, 10.1038/s44321-025-00197-4] PubMed: 39948420
Promoting the transition from pyroptosis to apoptosis in endothelial cells: a novel approach to alleviate methylglyoxal-induced vascular damage [ J Transl Med, 2025, 23(1):170] PubMed: 39930472
Hnf4α integrates AIF and caspase 3/9 signaling to restrict single and coinfecting pathogens in teleosts [ PLoS Pathog, 2025, 21(9):e1013491] PubMed: 40920830
3A and 2B proteins of SVA play chess game with host restriction factor DDX23 by apoptotic pathway [ J Virol, 2025, 99(10):e0076125] PubMed: 40956085

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.