Datasheet

Biological Description

Specificity	xCT Rabbit mAb recognizes endogenous levels of total xCT protein.
Background	xCT, also known as SLC7A11, is the light chain subunit of the cystine/glutamate antiporter system x_c^−, a sodium-independent transporter that facilitates a 1:1 exchange of extracellular cystine for intracellular glutamate across the plasma membrane. It is a multi-pass transmembrane protein that heterodimerizes with the heavy chain 4F2hc (SLC3A2) and adopts a conserved ‘5+5’ inverted topology fold, with a central binding site lined by positively charged residues that confer substrate specificity. The transport mechanism involves ligand-induced conformational changes, including partial unwinding of helices such as TM6B and TM8, to mediate alternating access to the binding site and ensure strict antiport activity. xCT is vital for redox homeostasis, as imported cystine is reduced to cysteine, the rate-limiting precursor for glutathione synthesis, thereby protecting cells from oxidative stress and ferroptosis. xCT is commonly upregulated in cancer, promoting tumor growth, survival, and resistance to therapy by maintaining intracellular redox balance.

Specificity

xCT Rabbit mAb recognizes endogenous levels of total xCT protein.

Background

xCT, also known as SLC7A11, is the light chain subunit of the cystine/glutamate antiporter system x_c^−, a sodium-independent transporter that facilitates a 1:1 exchange of extracellular cystine for intracellular glutamate across the plasma membrane. It is a multi-pass transmembrane protein that heterodimerizes with the heavy chain 4F2hc (SLC3A2) and adopts a conserved ‘5+5’ inverted topology fold, with a central binding site lined by positively charged residues that confer substrate specificity. The transport mechanism involves ligand-induced conformational changes, including partial unwinding of helices such as TM6B and TM8, to mediate alternating access to the binding site and ensure strict antiport activity. xCT is vital for redox homeostasis, as imported cystine is reduced to cysteine, the rate-limiting precursor for glutathione synthesis, thereby protecting cells from oxidative stress and ferroptosis. xCT is commonly upregulated in cancer, promoting tumor growth, survival, and resistance to therapy by maintaining intracellular redox balance.

Usage Information

Application

WB, IP, IHC, IF, FCM

Dilution

WB	IP	IHC	IF	FCM
1:1000	1:1000	1:500	1:500	1:50

Reactivity

Human, Mouse, Rat

Source

Rabbit

MW

55 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

xCT/SLC7A11 (H13H3) Rabbit mAb

Biological Description

Usage Information

References

Application Data