Vincristine

Catalog No.S9555 Batch:S955501

Technical Data

Formula	C₄₆H₅₆N₄O₁₀
Molecular Weight	824.96	CAS No.	57-22-7
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (121.21 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Vincristine (Leurocristine, NSC-67574, 22-Oxovincaleukoblastine) is a natural alkaloid isolated from the plant Vinca rosea Linn. with anti-tumor activity. Vincristine inhibits microtubule formation in mitotic spindle and binds to microtubule with Ki of 85 nM.
In vitro	Vincristine, a microtubule-destabilizing agent (MDA), inhibits the formation of microtubules and mitosis of the cancer cell, which is a victim of intratumoural metabolic resistance that has no important relevance with neither CYP3A4 nor CYP3A5.^[2]
In vivo	Vincristine shows the regression of the tumor in zebrafish xenografts.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HepG2_EV, HepG2_CYP3A4, HepG_CYP3A5, HepG2_CYP2C8 cells Concentrations 1 μM Incubation Time 24, 48 and 72 h Method HepG2 sublines (100×10⁴ cells/well) are seeded in 12-well plates and cultured until full confluence. Then, the media is removed, and the cells are exposed to 1 μM drug (docetaxel, paclitaxel or vincristine) or 0.01% DMSO (vehicle control). Samples are collected at 24, 48 and 72 h intervals using TRI Reagent, which is followed by RNA isolation. The RNA yield is assessed via a NanoDrop ND-1000 spectrophotometer, while its quality and integrity are verified by agarose gel electrophoresis.
Animal Study:^{^[3]}	Animal Models Zebrafish of glioma model Dosages 10-20 nM Administration p.o.

Cell Assay:^{^[2]}

Cell lines
HepG2_EV, HepG2_CYP3A4, HepG_CYP3A5, HepG2_CYP2C8 cells
Concentrations
1 μM
Incubation Time
24, 48 and 72 h
Method
HepG2 sublines (100×10⁴ cells/well) are seeded in 12-well plates and cultured until full confluence. Then, the media is removed, and the cells are exposed to 1 μM drug (docetaxel, paclitaxel or vincristine) or 0.01% DMSO (vehicle control). Samples are collected at 24, 48 and 72 h intervals using TRI Reagent, which is followed by RNA isolation. The RNA yield is assessed via a NanoDrop ND-1000 spectrophotometer, while its quality and integrity are verified by agarose gel electrophoresis.

Animal Study:^{^[3]}

Animal Models
Zebrafish of glioma model
Dosages
10-20 nM
Administration
p.o.

References

Selleck's Vincristine has been cited by 44 publications

Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer [ Sci Bull (Beijing), 2023, 10.1016/j.scib.2023.11.027]	PubMed: 37993335
Patient- and xenograft-derived organoids recapitulate pediatric brain tumor features and patient treatments [ EMBO Mol Med, 2023, 15(12):e18199]	PubMed: 38037472
TPX2 enhances the transcription factor activation of PXR and enhances the resistance of hepatocellular carcinoma cells to antitumor drugs [ Cell Death Dis, 2023, 14(1):64]	PubMed: 36707511
Targeting MCL-1 triggers DNA damage and an anti-proliferative response independent from apoptosis induction [ Cell Rep, 2023, 42(10):113176]	PubMed: 37773750
Benzimidazole carbamate induces cytotoxicity in breast cancer cells via two distinct cell death mechanisms [ Cell Death Discov, 2023, 9(1):162]	PubMed: 37179350
PTC596-Induced BMI-1 Inhibition Fights Neuroblastoma Multidrug Resistance by Inducing Ferroptosis [ Antioxidants (Basel), 2023, 13(1)3]	PubMed: 38275623
Monoclonal antibodies binding to different epitopes of CD20 differentially sensitize DLBCL to different classes of chemotherapy [ Front Oncol, 2023, 13:1159484]	PubMed: 37601699
FGF1 Protects MCF-7 Cells against Taltobulin through Both the MEKs/ERKs and PI3K/AKT Signaling Pathway [ Biomedicines, 2023, 11(7)1856]	PubMed: 37509496
Novel Biologically Active N-Substituted Benzimidazole Derived Schiff Bases: Design, Synthesis, and Biological Evaluation [ Molecules, 2023, 28(9)3720]	PubMed: 37175129
α-Mangostin Suppresses Melanoma Growth, Migration, and Invasion and Potentiates the Anti-tumor Effect of Chemotherapy [ Int J Med Sci, 2023, 20(9):1220-1234]	PubMed: 37575275

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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