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Biological Description
| Specificity | V5 Tag Antibody [L19E11] detects transfected levels of recombinant protein containing the V5 epitope tag. |
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| Background | The V5-tag is a short, 14–amino acid epitope tag originally derived from a prokaryotic source and widely used for recombinant protein detection and purification. Structurally, it contains an equal number of positively and negatively charged residues, yielding a pI of 5.85 and no net charge at physiological pH, with three proline residues that confer a partially ordered and stable core while remaining largely unstructured. Because of its small size, neutrality, and resistance to endogenous protease cleavage, the V5-tag can be fused to proteins without significantly altering their function or localization. It is commonly expressed in mammalian and insect cells without background interference and is detected using antibodies or nanobodies in assays such as ELISA, Western blotting, immunofluorescence, flow cytometry, immunoprecipitation, live-cell imaging, and protein–protein interaction studies. |
Usage Information
| Application | WB, IP, IF, ELISA | Dilution |
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| Reactivity | Tag | ||||||
| Source | Mouse Monoclonal Antibody | MW | 53 kDa | ||||
| Storage Buffer | PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3 | Storage (from the date of receipt) |
-20°C (avoid freeze-thaw cycles), 2 years | ||||
References
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