Ustekinumab (anti-IL-12/IL-23)

Catalog No.A2024        Batch: A202401

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Technical Data

CAS No. 815610-63-0
Formulation PBS, PH7.2
Isotype Human IgG1
Source Human
Storage
(From the date of receipt)
Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 99.12%
Protein concentration 5.00mg/ml
Endotoxin Level ≤1 EU/mg

Biological Activity

Description Ustekinumab (CNTO 1275) is an anti-IL-12/IL-23 IgG1κ human monoclonal antibody used to treat psoriasis. MW=145.6 kDa.
In vitro

The addition of Ustekinumab (UST) significantly inhibits T follicular helper (TFH) cell differentiation in vitro.[1]

In vivo

Ustekinumab is generated via recombinant human IL-12 immunization of human Ig (hu-Ig) transgenic mice. Ustekinumab binds to the p40 subunit common to IL-12 and IL-23 and prevents their interaction with the IL-12 receptor β1 subunit of the IL-12 and IL-23 receptor complexes.[2]

Protocol (Only for Reference)

Cell Assay:
  • Objective: TFH cell differentiation assay
    Cells: naïve CD4+ T cells, T follicular helper (TFH) cells, PBMCs
    Concentrations: 100 ng/mL
    Incubation Time: 24 h
    Method: The TFH cell differentiation assay is performed with freshly isolated PBMCs from healthy donors (n = 16). Naïve CD4+ T cells are isolated by using the human Naïve CD4+ T Cell Isolation Kit II. A total of 500,000 naïve CD4+ T cells are cultured in complete medium in the presence of 12.5 μL/mL anti-CD3/CD28 in a flat-bottom 48 well plate. After 24 hours of culture, cytokines are added and cells are incubated for additional 2.5 days. A total of 5 ng/mL IL-12 + 5 ng/mL TGFβ with and without 100 ng/mL UST and 100 ng/mL UST alone are used.
    Reference: https://pubmed.ncbi.nlm.nih.gov/32679193

    Ustekinumab can apply to humanized mice, peripheral blood and other related assays (Only for Reference)

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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