Tyrosinase Antibody [P8H17] Datasheet

Biological Description

Specificity	Tyrosinase Antibody [P8H17] detects endogenous levels of total Tyrosinase protein.
Background	Tyrosinase, the rate-limiting copper-containing monooxygenase of the tyrosinase family alongside TRP1 and TRP2, catalyzes the initial steps of melanogenesis by hydroxylating L-tyrosine to L-DOPA and oxidizing L-DOPA to dopaquinone through a type-3 dicopper active site featuring oxy-tyrosinase (Cu2O2) and met-tyrosinase (Cu2(OH)) intermediates. Binuclear copper coordination at His residues enables electrophilic aromatic attack, where dioxygen activation produces the μ-η2:η2-peroxo dicopper core that delivers the hydroxyl equivalent to the ortho position of monophenols, followed by two-electron oxidation of diphenols via substrate-assisted proton transfer; conformational shifts between syn/anti ligand geometries gate substrate access while latent zymogen activation by proteolysis exposes the catalytic cleft. The enzyme integrates within melanosomal complexes where TRP2 (DCT) accelerates dopachrome tautomerization, and TRP1 isomerizes quinone methides to 5,6-dihydroxyindoles, with MITF-driven transcriptional synergy at E-box/CATGTG motifs amplifying expression under UV/cAMP/PKA signaling. Tyrosinase governs constitutive and UV-inducible pigmentation in melanocytes, protecting basal keratinocytes from ROS while establishing retinal pigment epithelium barrier function critical for photoreceptor outer segment phagocytosis. OCA1A mutations abolish copper loading/trafficking, causing complete albinism with foveal hypoplasia, while hypomorphic OCA1B variants retain partial activity; conversely, melanoma exploits PKC-β phosphorylation and TPC2-mediated melanosome pH for hyperactivation, driving aberrant pigmentation that aids immunoevasion through melanin-mediated ROS quenching.

Specificity

Tyrosinase Antibody [P8H17] detects endogenous levels of total Tyrosinase protein.

Background

Tyrosinase, the rate-limiting copper-containing monooxygenase of the tyrosinase family alongside TRP1 and TRP2, catalyzes the initial steps of melanogenesis by hydroxylating L-tyrosine to L-DOPA and oxidizing L-DOPA to dopaquinone through a type-3 dicopper active site featuring oxy-tyrosinase (Cu2O2) and met-tyrosinase (Cu2(OH)) intermediates. Binuclear copper coordination at His residues enables electrophilic aromatic attack, where dioxygen activation produces the μ-η2:η2-peroxo dicopper core that delivers the hydroxyl equivalent to the ortho position of monophenols, followed by two-electron oxidation of diphenols via substrate-assisted proton transfer; conformational shifts between syn/anti ligand geometries gate substrate access while latent zymogen activation by proteolysis exposes the catalytic cleft. The enzyme integrates within melanosomal complexes where TRP2 (DCT) accelerates dopachrome tautomerization, and TRP1 isomerizes quinone methides to 5,6-dihydroxyindoles, with MITF-driven transcriptional synergy at E-box/CATGTG motifs amplifying expression under UV/cAMP/PKA signaling. Tyrosinase governs constitutive and UV-inducible pigmentation in melanocytes, protecting basal keratinocytes from ROS while establishing retinal pigment epithelium barrier function critical for photoreceptor outer segment phagocytosis. OCA1A mutations abolish copper loading/trafficking, causing complete albinism with foveal hypoplasia, while hypomorphic OCA1B variants retain partial activity; conversely, melanoma exploits PKC-β phosphorylation and TPC2-mediated melanosome pH for hyperactivation, driving aberrant pigmentation that aids immunoevasion through melanin-mediated ROS quenching.

Usage Information

Application

WB, IHC

Dilution

WB	IHC
1:1000-1:5000	1:100-1:500

Reactivity

Mouse, Human

Source

Rabbit Monoclonal Antibody

MW

60 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/30596633/
https://pubmed.ncbi.nlm.nih.gov/7969144/

Application Data

WB

Validated by Selleck

Lane 1: Mewo