Trypan Blue

Catalog No.S6890 Batch:S689001

Technical Data

Formula	C₃₄H₂₄N₆Na₄O₁₄S₄
Molecular Weight	960.81	CAS No.	72-57-1
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (104.07 mM)
		Water	100 mg/mL (104.07 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Trypan Blue (Visionblue, Diphenyl Blue, Direct Blue 14) is a colorimetric dye that stains dead cells with a blue color easily observed using light microscopy at low resolution.
In vitro	1.1 Preparation of the stock solution Dissolve 10 mg of Trypan Blue in 100 mL 0.85% Nacl to obtain 0.4% Trypan Blue. Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of Trypan Blue working solution Dilute the stock solution in 0.85% Nacl to obtain 0.04% of Trypan Blue working solution. Note: Please adjust the concentration of Trypan Blue working solution according to the actual situation. Cell staining 2.1 For suspension cells, centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells, discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2.2 Add 1 mL of rypan Blue working solution, and then incubate at room temperature for 5 minutes. 2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. 2.4 Wash twice with PBS, 5 minutes each time. 2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope.

Description

Trypan Blue (Visionblue, Diphenyl Blue, Direct Blue 14) is a colorimetric dye that stains dead cells with a blue color easily observed using light microscopy at low resolution.

In vitro

1.1 Preparation of the stock solution
Dissolve 10 mg of Trypan Blue in 100 mL 0.85% Nacl to obtain 0.4% Trypan Blue.
Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Trypan Blue working solution
Dilute the stock solution in 0.85% Nacl to obtain 0.04% of Trypan Blue working solution.
Note: Please adjust the concentration of Trypan Blue working solution according to the actual situation.
Cell staining
2.1 For suspension cells, centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells, discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of rypan Blue working solution, and then incubate at room temperature for 5 minutes.
2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope.

Protocol (from reference)

References

[1] Lisa C Crowley, et al. Cold Spring Harb Protoc. 2016 Jul 1;2016(7).

Selleck's Trypan Blue has been cited by 1 publication

Main Determinants Affecting the Antiproliferative Activity of Stilbenes and Their Gut Microbiota Metabolites in Colon Cancer Cells: A Structure-Activity Relationship Study [ Int J Mol Sci, 2022, 23(23)15102]	PubMed: 36499424

Main Determinants Affecting the Antiproliferative Activity of Stilbenes and Their Gut Microbiota Metabolites in Colon Cancer Cells: A Structure-Activity Relationship Study [ Int J Mol Sci, 2022, 23(23)15102]

PubMed: 36499424

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.