TLR2-IN-C29

Catalog No.S6597 Batch:S659701

Technical Data

Formula	C₁₆H₁₅NO₄
Molecular Weight	285.29	CAS No.	363600-92-4
Solubility (25°C)*	In vitro	DMSO	57 mg/mL (199.79 mM)
		Ethanol	6 mg/mL (21.03 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

TLR2-IN-C29 (C29) is a Toll-like receptor 2 (TLR2) inhibitor that inhibits TLR2/1 and TLR2/6 signaling.

Targets

TLR2 ^[1]

In vitro

TLR2-IN-C29 inhibites TLR2/1 and TLR2/6 signaling induced by synthetic and bacterial TLR2 agonists in human HEK-TLR2 and THP-1 cells, but only TLR2/1 signaling in murine macrophages. TLR2-IN-C29 fails to inhibit signaling induced by other TLR agonists and TNF-α.^[2]

In vivo

TLR2-IN-C29 reduces significantly the growth of tumors in Clear cell Renal Cell (RCC) and Head and Neck Squamous Cell Carcinomas (HNSCC) xenograft mice.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines THP-1 cells, HEK-TLR2 cells, murine macrophages Concentrations 10 μM-150 μM Incubation Time 5 h Method After transfection, cells are allowed to recover for 48 h and treated for 5 h with medium or stimuli in the presence/absence of C29. Cells are lysed in a passive lysis buffer, and firefly luciferase and Renilla luciferase activities are measured using the Dual-Luciferase Reporter Assay System. Cytokine levels in culture supernatants are analyzed by Multiplex beads in the Cytokine Core Laboratory.

Cell Assay:^{^[2]}

Cell lines
THP-1 cells, HEK-TLR2 cells, murine macrophages
Concentrations
10 μM-150 μM
Incubation Time
5 h
Method
After transfection, cells are allowed to recover for 48 h and treated for 5 h with medium or stimuli in the presence/absence of C29. Cells are lysed in a passive lysis buffer, and firefly luciferase and Renilla luciferase activities are measured using the Dual-Luciferase Reporter Assay System. Cytokine levels in culture supernatants are analyzed by Multiplex beads in the Cytokine Core Laboratory.

References

Selleck's TLR2-IN-C29 has been cited by 7 publications

An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression [ Cell Res, 2023, 33(8):585-603]	PubMed: 37337030
Dual RNA-Sequencing and Liquid Chromatography-Mass Spectrometry Unveil Specific Insights on the Pathogenicity of Trichophyton mentagrophytes Complex [ J Invest Dermatol, 2023, 143(3):470-479.e6]	PubMed: 38295003
Acrylamide induces the activation of BV2 microglial cells through TLR2/4-mediated LRRK2-NFATc2 signaling cascade [ Food Chem Toxicol, 2023, 176:113775]	PubMed: 37037409
Acrylamide induces the activation of BV2 microglial cells through TLR2/4-mediated LRRK2-NFATc2 signaling cascade [ Food Chem Toxicol, 2023, 176:113775]	PubMed: 37037409
Airway Prevotella promote TLR2-dependent neutrophil activation and rapid clearance of Streptococcus pneumoniae from the lung [ Nat Commun, 2022, 13(1):3321]	PubMed: 35680890
Distinct activities of Vδ1+ T-cells upon different cytomegalovirus reactivation status after haematopoietic transplantation [ Immunology, 2022, 167(3):368-383]	PubMed: 35795896
Structural characterization and immunomodulatory mechanisms of two novel glucans from Morchella importuna fruiting bodies [ Int J Biol Macromol, 2021, 183:145-157]	PubMed: 33878360

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SHIPPING AND STORAGE
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