TLQP-21 TFA

Catalog No.P1209 Batch:P120902

Technical Data

Formula	C₁₀₇H₁₇₀N₄₀O₂₆(TFA)
Molecular Weight	2546.75	CAS No.	869988-94-3(free base)
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (39.26 mM)
		Water	100 mg/mL (39.26 mM)
		Ethanol	100 mg/mL (39.26 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

LQP-21 TFA (TLQP-21 Trifluoroacetate) is a VGF-derived peptide that activates the C3aR1 receptor through a folding-upon-binding mechanism.

Targets

C3aR1 receptor ^[1]

In vitro

TLQP-21 activates the cognate C3aR1 receptor leading to enhanced lipolysis in adipocytes and arginine 21 is essential for TLQP-21 lipolysis in 3T3L1 adipocytes.^[1]

Density

95.0~105.0%

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines 3T3L1 adipocytes Concentrations 100 nM Incubation Time 3 h Method 3T3-L1 adipocytes differentiated on 6-well plates are first starved for 3 hours in KRH with 1% BSA, then incubate with TLQP-21 in the presence and absence of specific agonists/antagonists, suspended in KRH with 4% BSA. Following 3 hours of incubation at 37°C and 5% CO2, the media is collected and incubated at 60°C for 20 min to inactivate any residual enzymatic reaction activity. The cells are gathered for protein concentration or trypsinized for cell diameter and viability measurements.

Cell Assay:^{^[1]}

Cell lines
3T3L1 adipocytes
Concentrations
100 nM
Incubation Time
3 h
Method
3T3-L1 adipocytes differentiated on 6-well plates are first starved for 3 hours in KRH with 1% BSA, then incubate with TLQP-21 in the presence and absence of specific agonists/antagonists, suspended in KRH with 4% BSA. Following 3 hours of incubation at 37°C and 5% CO2, the media is collected and incubated at 60°C for 20 min to inactivate any residual enzymatic reaction activity. The cells are gathered for protein concentration or trypsinized for cell diameter and viability measurements.

References

[1] Cheryl Cero, et al. Structure . 2014 Dec 2;22(12):1744-1753.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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