TIPE2 Antibody [A15H13] Datasheet

Biological Description

Specificity	TIPE2 Antibody [A15H13] detects endogenous levels of total TIPE2 protein.
Background	TNF‑α–induced protein 8–like 2 (TIPE2; TNFAIP8L2) is a cytosolic immune‑regulatory protein of the TNFAIP8/TIPE family that functions as a negative modulator of innate and adaptive immune signaling in myeloid and lymphoid cells. The protein is constitutively expressed in macrophages, NK cells, and T lymphocytes and does not form a stable, pre‑assembled complex but instead engages membrane‑proximal and signaling‑adapter molecules to restrain NF‑κB and MAPK pathway activation. TIPE2 interacts with and dampens the activity of key kinases and adaptors in these cascades, including components that regulate IKK and JNK, thereby limiting the production of pro‑inflammatory cytokines and preventing excessive immune activation under homeostatic and challenge conditions. Reduced TIPE2 expression or loss‑of‑function exacerbates inflammatory pathology, as seen in models of colitis and arthritis, whereas forced overexpression of TIPE2 suppresses inflammatory signaling and ameliorates tissue damage, acting as a checkpoint in immune‑mediated diseases. TIPE2 exerts a dual, context‑dependent function, inhibiting antitumor immunity in some solid‑tumor microenvironments by enhancing immunosuppressive cell populations and dampening cytotoxic responses, yet in certain colitis‑associated cancer models, it can act as a colonocyte‑intrinsic tumor suppressor by restraining inflammation‑driven proliferation.

Specificity

TIPE2 Antibody [A15H13] detects endogenous levels of total TIPE2 protein.

Background

TNF‑α–induced protein 8–like 2 (TIPE2; TNFAIP8L2) is a cytosolic immune‑regulatory protein of the TNFAIP8/TIPE family that functions as a negative modulator of innate and adaptive immune signaling in myeloid and lymphoid cells. The protein is constitutively expressed in macrophages, NK cells, and T lymphocytes and does not form a stable, pre‑assembled complex but instead engages membrane‑proximal and signaling‑adapter molecules to restrain NF‑κB and MAPK pathway activation. TIPE2 interacts with and dampens the activity of key kinases and adaptors in these cascades, including components that regulate IKK and JNK, thereby limiting the production of pro‑inflammatory cytokines and preventing excessive immune activation under homeostatic and challenge conditions. Reduced TIPE2 expression or loss‑of‑function exacerbates inflammatory pathology, as seen in models of colitis and arthritis, whereas forced overexpression of TIPE2 suppresses inflammatory signaling and ameliorates tissue damage, acting as a checkpoint in immune‑mediated diseases. TIPE2 exerts a dual, context‑dependent function, inhibiting antitumor immunity in some solid‑tumor microenvironments by enhancing immunosuppressive cell populations and dampening cytotoxic responses, yet in certain colitis‑associated cancer models, it can act as a colonocyte‑intrinsic tumor suppressor by restraining inflammation‑driven proliferation.

Usage Information

Application

WB, IP, IHC, IF

Dilution

WB	IP	IHC	IF
1:1000	1:30	1:100	1:50

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

20 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/31616442/
https://pubmed.ncbi.nlm.nih.gov/38377476/

Application Data

WB

Validated by Selleck

Lane 1: THP-1