TIM 1 Antibody [M14A22] Datasheet

Biological Description

Specificity	TIM 1 Antibody [M14A22] detects endogenous levels of total TIM 1 protein.
Background	TIM‑1 (T‑cell immunoglobulin and mucin domain‑1, HAVCR1) is a type I transmembrane receptor of the TIM family that is expressed on activated CD4⁺ T cells, B cells, invariant NKT cells, and some epithelial cells, where it regulates both adaptive and innate immune responses. The extracellular region contains an Ig‑like domain with a metal‑ion–dependent ligand‑binding site that recognizes phosphatidylserine on apoptotic cell membranes, followed by a heavily glycosylated mucin stalk, while the short cytoplasmic tail carries tyrosine and serine residues that connect TIM‑1 engagement to intracellular signaling cascades. Interaction of TIM‑1 with phosphatidylserine on apoptotic cells or with agonistic antibodies enhances CD4⁺ T‑cell activation, increases expression of Th2‑associated transcription factors, and promotes production of IL‑4, IL‑5, IL‑10, and IL‑13, linking TIM‑1 ligation to skewing of T‑helper differentiation toward Th2 profiles. TIM‑1 expression on T cells is upregulated under Th2‑skewing conditions, that TIM‑1 costimulation augments Th2 cytokine responses and airway eosinophilia, and that genetic or antibody‑mediated interference with TIM‑1 reduces Th2 cytokines and airway inflammation. TIM‑1 is also expressed on invariant NKT cells, where engagement in the presence of TCR stimulation enhances IL‑4 production and reduces IFN‑γ, adding a second route by which TIM‑1 biases effector programs toward type 2 immunity and contributes to airway hyperreactivity after recognition of apoptotic cells.

Specificity

TIM 1 Antibody [M14A22] detects endogenous levels of total TIM 1 protein.

Background

TIM‑1 (T‑cell immunoglobulin and mucin domain‑1, HAVCR1) is a type I transmembrane receptor of the TIM family that is expressed on activated CD4⁺ T cells, B cells, invariant NKT cells, and some epithelial cells, where it regulates both adaptive and innate immune responses. The extracellular region contains an Ig‑like domain with a metal‑ion–dependent ligand‑binding site that recognizes phosphatidylserine on apoptotic cell membranes, followed by a heavily glycosylated mucin stalk, while the short cytoplasmic tail carries tyrosine and serine residues that connect TIM‑1 engagement to intracellular signaling cascades. Interaction of TIM‑1 with phosphatidylserine on apoptotic cells or with agonistic antibodies enhances CD4⁺ T‑cell activation, increases expression of Th2‑associated transcription factors, and promotes production of IL‑4, IL‑5, IL‑10, and IL‑13, linking TIM‑1 ligation to skewing of T‑helper differentiation toward Th2 profiles. TIM‑1 expression on T cells is upregulated under Th2‑skewing conditions, that TIM‑1 costimulation augments Th2 cytokine responses and airway eosinophilia, and that genetic or antibody‑mediated interference with TIM‑1 reduces Th2 cytokines and airway inflammation. TIM‑1 is also expressed on invariant NKT cells, where engagement in the presence of TCR stimulation enhances IL‑4 production and reduces IFN‑γ, adding a second route by which TIM‑1 biases effector programs toward type 2 immunity and contributes to airway hyperreactivity after recognition of apoptotic cells.

Usage Information

Application

WB, IP, IHC, IF, FCM

Dilution

WB	IP	IHC	IF	FCM
1:1000	1:30	1:2000	1:100	1:500

Reactivity

Mouse

Source

Rabbit Monoclonal Antibody

MW

39 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/18706830/
https://pubmed.ncbi.nlm.nih.gov/22144095/

TIM 1 Antibody [M14A22]

Biological Description

Usage Information

References

Application Data