SW033291

Catalog No.S7900 Batch:S790001

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Technical Data

Formula

C21H20N2OS3
Molecular Weight 412.59 CAS No. 459147-39-8
Solubility (25°C)* In vitro DMSO 83 mg/mL (201.16 mM)
Ethanol 32 mg/mL (77.55 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description SW033291 is a selective inhibitor of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) with IC50 and Ki of 1.5 nM and 0.1 nM, respectively.
Targets
15-PGDH [1]
(Cell-free assay)		 15-PGDH [1]
(Cell-free assay)
0.1 nM(Ki) 1.5 nM
In vitro

In Vaco-503 cells, SW033291 (2.5 μM) decreases cellular 15-PGDH enzyme activity by 85%.[1]
In vivo

In mice receiving a bone marrow transplant, SW033291 (10 mg/kg, i.p.) promotes hematopoietic recovery. In mouse models of colon and liver injury, SW033291 (10 mg/kg, i.p.) reduces the levels of colitis-associated inflammatory cytokines, protects mice from colitis, and promotes liver regeneration. [1]

Protocol (from reference)

Kinase Assay:

[1]
  • Activity assays of recombinant 15-PGDH protein

    For initial characterization of inhibition of 15-PGDH enzyme activity by SW033291, reactions are assembled with experiment specific concentrations of 15-PGDH enzyme, and experiment specific concentrations of SW033291, plus 150 μM NAD(+) and 25 μM PGE2 in reaction buffer (50 mM Tris-HCl, pH7.5, 0.01% Tween 20). The reaction mix is incubated for 15 min at 25 ºC in an Envision Reader. Enzyme activity is determined by following generation of NADH as assayed by recording fluorescence at Ex/Em=340 nM/485 nM every 30s for 3 minutes, commencing immediately after addition of PGE2. IC50 values are calculated with GraphPad Prism 5 software using the sigmoidal dose-response function and plotted against SW033291 concentration.
Cell Assay:

[2]
  • Cell lines

    BMSCs

  • Concentrations

    1 μM

  • Incubation Time

    5 days

  • Method

    Cells were treated with indicated concentration of drug for 5 days.
Animal Study:

[1]
  • Animal Models

    Mice receiving a bone marrow transplant, and Mouse models of colon and liver injury

  • Dosages

    10 mg/kg twice daily

  • Administration

    i.p.

Selleck's SW033291 has been cited by 5 publications

Divalent metal cations stimulate skeleton interoception for new bone formation in mouse injury models [ Nat Commun, 2022, 13(1):535] PubMed: 35087048
Skeleton interoception regulates bone and fat metabolism through hypothalamic neuroendocrine NPY [ Elife, 2021, 10e70324] PubMed: 34468315
3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization [ Pharmaceutics, 2021, 13(11)1934] PubMed: 34834349
Sensory nerves regulate mesenchymal stromal cell lineage commitment by tuning sympathetic tones. [ J Clin Invest, 2020, 131554] PubMed: 32191640
Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis. [ Nat Commun, 2019, 10(1):181] PubMed: 30643142

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.