G10 (STING agonist-1)

Catalog No.S8954 Batch:S895401

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Technical Data

Formula

C21H16ClFN2O3S
Molecular Weight 430.88 CAS No. 702662-50-8
Solubility (25°C)* In vitro DMSO 63 mg/mL (146.21 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 1.050mg/ml (2.44mM) Taking the 1 mL working solution as an example, add 50 μL of 21 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution
5% DMSO 95% Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 1.050mg/ml (2.44mM) Taking the 1 mL working solution as an example, add 50 μL of 21 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description G10 (STING agonist-1) is a novel human-specific STING agonist that triggers IFN regulatory factor 3 (IRF3)/ type I interferon (IFN)-associated transcription in human fibroblasts. This compound potently reduces growth of Chikungunya virus (CHIKV) with IC90 of 8.01 μM and blocks replication of Alphavirus species Venezuelan Equine Encephalitis Virus (VEEV) with IC90 of 24.57 μM.
Targets
STING [1] IRF3/IFN [1] CHIKV [1]
(Cell-based assay)		 VEEV [1]
(Cell-based assay)
8.01 μM 24.57 μM
In vitro

G10 (STING agonist-1) induces IFN/IRF3- but not NF-κB-dependent transcription in human fibroblasts, and elicits antiviral activity against New and Old World Alphaviruses. It triggers STING-dependent synthesis and secretion of bioactive interferon, induces IRF3-dependent activity, and promotes innate antiviral mRNA expression in primary human cells.[1]
In vivo

Because G10 (STING agonist-1)-induced innate immune activation is not observed in murine cells, DMXAA (an analog of this compound, with similar physiological effects) is used in the commonly used mice model of CHIKV infection. STING agonism inhibits CHIKV replication in vivo.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Human foreskin fibroblasts, Vero cells, BHK-21 cells, C6/36 cells, RAW264.7 cells, THP1-ISG-Lucia cells, Human peripheral blood mononuclear cells

  • Concentrations

    6.25-200 μM, 100 μM

  • Incubation Time

    7 h, 18 h

  • Method

    --

References

  • https://pubmed.ncbi.nlm.nih.gov/26646986/

Selleck's G10 (STING agonist-1) Has Been Cited by 1 Publication

Lats2 deficiency protects the heart against myocardial infarction by reducing inflammation and inhibiting mitochondrial fission and STING/p65 signaling [ Int J Biol Sci, 2023, 19(11):3428-3440] PubMed: 37497006

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.