SRI-011381 (C381)

Catalog No.S6654 Batch:S665401

Technical Data

Formula

C₂₀H₃₁N₃O

Molecular Weight

329.48

CAS No.

1629138-41-5

Solubility (25°C)*

In vitro

DMSO

66 mg/mL (200.31 mM)

Ethanol

66 mg/mL (200.31 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.3mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 66 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	3.3mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 66 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

SRI-011381 (C381) is a novel agonist of the TGF-beta signaling pathway for treatment of Alzheimer's disease.C381 physically targets the lysosome, promotes lysosomal acidification, increases breakdown of lysosomal cargo, and improves lysosome resilience to damage.

Targets

TGF-β ^[1]

In vitro

SRI-011381 partially reverses the effect of sodium ferulate (SF) on the expression of fibrosis-related proteins, by increasing TGF-β1, NALP3, collagen-1, and α-SMA expression.^[2]

In vivo

SRI-011381, a small-molecule agonist of TGFβ signaling, has no effect on outflow tract (OFT) expansion in zebrafish, suggesting that hyperactivation of TGFβ signaling is not sufficient to induce aneurysm.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines pulmonary fibroblasts of silicosis mouse model Concentrations 10 µM Incubation Time -- Method The tissues are extracted in a sterile environment, rinsed in PBS 3 times, and then cut into 1-mm³ tissue blocks. After repeated digestion with 0.25% trypsin at 37 ℃, the histolysate is evenly spread in a 10-cm petri dish. A high-glucose medium containing 10% fetal bovine serum, penicillin, and streptomycin is added to the petri dish. The cells are cultured in an incubator, and the medium is exchanged every 2-3 days. When the cells reached 80-90% confluence, they are passaged according to the ratio of 1: 3, and the fifth passage cells are taken for experimental study. Pulmonary fibroblasts are identified using anti-vimentin antibody with fluorescent secondary antibodies under a fluorescence microscope. For in vitro experiments, a range of concentrations of SF solution (0.04, 0.1, 0.18 and 0.28 mg/mL) are prepared for the treatment of fibroblasts. A 10 μM SRI-011381 solution is used for the activation of TGF-β1 signaling.
Animal Study:^{^[3]}	Animal Models Zebrafish carrying ltbp3^fb28 and ltbp1^fb29 Dosages 500 µM Administration exposed

Cell Assay:^{^[2]}

Cell lines
pulmonary fibroblasts of silicosis mouse model
Concentrations
10 µM
Incubation Time
--
Method
The tissues are extracted in a sterile environment, rinsed in PBS 3 times, and then cut into 1-mm³ tissue blocks. After repeated digestion with 0.25% trypsin at 37 ℃, the histolysate is evenly spread in a 10-cm petri dish. A high-glucose medium containing 10% fetal bovine serum, penicillin, and streptomycin is added to the petri dish. The cells are cultured in an incubator, and the medium is exchanged every 2-3 days. When the cells reached 80-90% confluence, they are passaged according to the ratio of 1: 3, and the fifth passage cells are taken for experimental study. Pulmonary fibroblasts are identified using anti-vimentin antibody with fluorescent secondary antibodies under a fluorescence microscope. For in vitro experiments, a range of concentrations of SF solution (0.04, 0.1, 0.18 and 0.28 mg/mL) are prepared for the treatment of fibroblasts. A 10 μM SRI-011381 solution is used for the activation of TGF-β1 signaling.

Animal Study:^{^[3]}

Animal Models
Zebrafish carrying ltbp3^fb28 and ltbp1^fb29
Dosages
500 µM
Administration
exposed

References

[4] Vest RT, et al. Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2121609119.

+ Expand

Selleck's SRI-011381 (C381) has been cited by 4 publications

Latent TGFβ binding proteins 1 and 3 protect the larval zebrafish outflow tract from aneurysmal dilatation [ Dis Model Mech, 2022, dmm.046979]	PubMed: 35098309
Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes [ Front Cell Dev Biol, 2021, 9:648201]	PubMed: 33748142
MicroRNA-210-5p alleviates cardiac fibrosis via targeting transforming growth factor-beta type I receptor in rats on high sodium chloride (NaCl)-based diet [ Eur J Pharmacol, 2021, 912:174587]	PubMed: 34678242
MMP11 promotes the proliferation and progression of breast cancer through stabilizing Smad2 protein [ Oncol Rep, 2021, 45(4)16]	PubMed: 33649832

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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