SR-4835

Catalog No.S8894 Batch:S889401

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Technical Data

Formula

C21H20Cl2N10O
Molecular Weight 499.36 CAS No. 2387704-62-1
Solubility (25°C)* In vitro DMSO 60 mg/mL (120.15 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description SR-4835 is a highly selective dual inhibitor of CDK12 and CDK13 with IC50 of 99 nM and Kd of 98 nM for CDK12 and IC50 of 4.9 nM for CDK13. This compound disables triple-negative breast cancer (TNBC) cells. It promotes synergy with DNA-damaging chemotherapy and PARP inhibitors.
Targets
CDK13 [1]
(Cell-free assay)		 CDK12 [1]
(Cell-free assay)		 CDK12 [1]
(Cell-free assay)
4.9 nM 98 nM(Kd) 99 nM
In vitro

SR-4835 is a selective, potent dual inhibitor of CDK12 and CDK13, suppressing expression of DDR proteins. This compound's inhibition synergizes with DNA-damaging agents or PARP inhibition to trigger TNBC cell death.[1]
In vivo

SR-4835 has potent in vivo anti-TNBC activity and augments the anti-cancer activity. This compound is well tolerated in mice after long-term dosing. It cooperates with DNA-damaging chemotherapeutics.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Human: MDA-MB-231, MDA-MB-436, HS578T, MDA-MB-468, FHC

  • Concentrations

    10-90 nM, 0.4-10 μM

  • Incubation Time

    4 h, 6 h, 72 h

  • Method

    Clonogenic Assays. 500 cells per well are plated in six-well dishes in triplicate. After overnight incubation, SR-4835 is added to the medium for 72 hr, and cells are allowed to grow for 7 to 10 days, during which medium is changed every 2 to 3 days.
Animal Study:

[1]
  • Animal Models

    Female SCID Beige mice bearing xenograft tumor fragments, male C57Bl-6 mice

  • Dosages

    20 mg/kg

  • Administration

    Oral gavage, IV

References

  • https://pubmed.ncbi.nlm.nih.gov/31668947/

Selleck's SR-4835 Has Been Cited by 2 Publications

CDK12 regulates cellular metabolism to promote glioblastoma growth [ JCI Insight, 2025, 10(21)e190780] PubMed: 40996961
Molecular consequences of acute versus chronic CDK12 loss in prostate carcinoma nominates distinct therapeutic strategies [ bioRxiv, 2024, 2024.07.16.603734] PubMed: 39071291

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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