SAG (Smoothened Agonist)

Catalog No.S6384 Batch:S638402

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Technical Data

Formula

C28H28ClN3OS
Molecular Weight 490.06 CAS No. 912545-86-9
Solubility (25°C)* In vitro DMSO 98 mg/mL (199.97 mM)
Ethanol 98 mg/mL (199.97 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description SAG (Smoothened Agonist) is a potent agonist of Smoothened (Smo). Smoothened Agonist induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM.
Targets
Smo [1]
(in Shh-LIGHT2 cells)
3 nM(EC50)
In vitro

SAG can counteract glucocorticoids (GCs) antagonism of Shh-driven granule neuron precursor (CGNP) proliferation.[2]
In vivo

Systemic administration of Smoothened Agonist (SAG) prevents the neurotoxic effects of glucocorticoids. Smoothened Agonist can cross the blood-brain barrier to activate Shh transcriptional targets in vivo. SAG at the treatment dose effectively prevents GC-induced neonatal cerebellar developmental abnormalities.[2]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Granule neuron precursor (CGNP) primary cells

  • Concentrations

    15-240 nM

  • Incubation Time

    24 h

  • Method

    CGNP are stimulated by various SAG concentrations (15 to 240 nM) compared with ShhN (3 μg/ml) and vehicle after 24 hours in vitro. Quantitative reverse transcription–PCR is performed with SYBR Green master mix in LightCycler 480.

Selleck's SAG (Smoothened Agonist) has been cited by 16 publications

Identification of a distal enhancer regulating hedgehog interacting protein gene in human lung epithelial cells [ EBioMedicine, 2024, 101:105026] PubMed: 38417378
Sonic hedgehog restrains the ubiquitin-dependent degradation of SP1 to inhibit neuronal/glial senescence associated phenotypes in chemotherapy-induced peripheral neuropathy via the TRIM25-CXCL13 axis [ J Adv Res, 2024, S2090-1232(24)00106-1] PubMed: 38479571
Single-cell RNA sequencing reveals the developmental program underlying proximal-distal patterning of the human lung at the embryonic stage [ Cell Res, 2023, 33(6):421-433] PubMed: 37085732
Highly reproducible and cost-effective one-pot organoid differentiation using a novel platform based on PF-127 triggered spheroid assembly [ Biofabrication, 2023, 15(4).] PubMed: 37552975
Triptolide inhibits epithelial ovarian tumor growth by blocking the hedgehog/Gli pathway [ Aging (Albany NY), 2023, 10.18632/aging.205110] PubMed: 37851362
Primary cilia support cartilage regeneration after injury [ Int J Oral Sci, 2023, 15(1):22] PubMed: 37268650
Protocol for the rapid intravenous in ovo injection of developing amniote embryos [ STAR Protoc, 2023, 4(2):102324] PubMed: 37210721
Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content [ Nat Commun, 2022, 13(1):2387] PubMed: 35501322
The PROTAC selectively degrading Bcl-xL represents a novel Hedgehog pathway inhibitor with capacity of combating resistance to Smoothened inhibitors while sparing bone growth [ Theranostics, 2022, 12(17):7476-7490] PubMed: 36438482
CK2 blockade alleviates liver fibrosis by suppressing activation of hepatic stellate cells via the Hedgehog pathway [ Br J Pharmacol, 2022, 10.1111/bph.15945] PubMed: 36070072

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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