Sec31A Antibody [J15M23] Datasheet

Biological Description

Specificity	Sec31A Antibody [J15M23] detects endogenous levels of total Sec31A protein.
Background	Sec31A is a core subunit of the coat protein complex II (COPII), a multimeric coat that mediates the formation of transport vesicles at the interface between the endoplasmic reticulum and the Golgi apparatus. Sec31A forms a β‑propeller WD‑repeat domain that interacts with Sec13 and self‑assembles into flexible, cage‑like scaffolds that physically shape budding COPII vesicles and simultaneously stimulate the GTPase activity of the Sar1–Sec23/24 inner coat complex, thereby coordinating the recruitment, deformation, and fission of ER‑derived carriers. Sec31A also contains a proline‑rich C‑terminal region that binds regulatory proteins and calcium‑sensing factors such as ALG‑2 and annexin‑A11, enabling luminal–cytosolic crosstalk and Ca²⁺‑dependent modulation of coat stability and vesicle‑budding kinetics, and its ubiquitously expressed isoform contrasts with the more restricted Sec31B paralog, whose expression peaks in brain and testis. Sec31A helps couple COPII assembly to ER‑export signals, ensuring that nascent secretory and membrane proteins are packaged into ER‑to‑Golgi carriers, and disruption of Sec31A function delays cargo exit, perturbs ER‑Golgi trafficking, and impairs secretion and cell‑surface delivery. Chromosomal rearrangements generate an SEC31A–JAK2 fusion that drives constitutive JAK2 kinase activity and downstream STAT‑dependent signaling, rendering cells sensitive to JAK inhibitors.

Specificity

Sec31A Antibody [J15M23] detects endogenous levels of total Sec31A protein.

Background

Sec31A is a core subunit of the coat protein complex II (COPII), a multimeric coat that mediates the formation of transport vesicles at the interface between the endoplasmic reticulum and the Golgi apparatus. Sec31A forms a β‑propeller WD‑repeat domain that interacts with Sec13 and self‑assembles into flexible, cage‑like scaffolds that physically shape budding COPII vesicles and simultaneously stimulate the GTPase activity of the Sar1–Sec23/24 inner coat complex, thereby coordinating the recruitment, deformation, and fission of ER‑derived carriers. Sec31A also contains a proline‑rich C‑terminal region that binds regulatory proteins and calcium‑sensing factors such as ALG‑2 and annexin‑A11, enabling luminal–cytosolic crosstalk and Ca²⁺‑dependent modulation of coat stability and vesicle‑budding kinetics, and its ubiquitously expressed isoform contrasts with the more restricted Sec31B paralog, whose expression peaks in brain and testis. Sec31A helps couple COPII assembly to ER‑export signals, ensuring that nascent secretory and membrane proteins are packaged into ER‑to‑Golgi carriers, and disruption of Sec31A function delays cargo exit, perturbs ER‑Golgi trafficking, and impairs secretion and cell‑surface delivery. Chromosomal rearrangements generate an SEC31A–JAK2 fusion that drives constitutive JAK2 kinase activity and downstream STAT‑dependent signaling, rendering cells sensitive to JAK inhibitors.

Usage Information

Application

WB, IF

Dilution

WB	IF
1:1000	1:400

Reactivity

Human, Mouse, Rat, Monkey

Source

Rabbit Monoclonal Antibody

MW

95-140 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/21325169/

Application Data

WB

Validated by Selleck

Lane 1: MCF7, Lane 2: SKOV-3, Lane 3: K562