S(-)-Propranolol hydrochloride

Catalog No.S5981 Batch:S598101

Technical Data

Formula	C₁₆H₂₂ClNO₂
Molecular Weight	295.8	CAS No.	4199-10-4
Solubility (25°C)*	In vitro	DMSO	59 mg/mL (199.45 mM)
		Water	59 mg/mL (199.45 mM)
		Ethanol	20 mg/mL (67.61 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	(S)-(-)-Propranolol hydrochloride, the active enantiomer of propranolol, is a β-Adrenoceptor (β-Adrenergic Receptor) antagonist with log Kd values of -8.16, -9.08, and -6.93 for β1, β2, and β3, respectively.
In vitro	Propranolol, a non-selective β1 and β2 adrenergic blocker, has no effect on the proliferation of neonatal mouse cardiac fibroblasts (NMCFs).^[2]
In vivo	Propranolol, an anti-arrhythmic drugs, consistently reduces the heart rates of adult mice by approximately 10%-20%, whereas isoproterenol has the opposite effect.^[2]

Protocol (from reference)

Cell Assay:^[2]	Cell lines neonatal mice cardiomyocytes (NMCMs) Concentrations 10, 20, 40, 50, and 100 μM Incubation Time 6, 12, 18, and 24 h Method In vitro, the neonatal mice cardiomyocytes (NMCMs) are treated with different drugs of different concentration, including isoproterenol (0, 1, 2.5, 5, 10, 20, and 50 μM); amiodarone (0, 1, 2.5, 5, 10, 20, and 50 μM); metoprolol (0, 10, 20, 30, 50, 100, 150, and 200 μM); propranolol (0, 10, 20, 40, 50, and 100 μM); lidocaine (0, 1, 2.5, 5, 10, 20, and 50 μM); verapamil(0, 1, 2.5, 5, 10, 20, and 50 μM);ivabradine (0, 1, 2, 3, 5, 10, and 20 μM). And the concentrations of drugs that promote CMs proliferation most significantly are chosen for subsequent experiments, including isoproterenol (10 μM), amiodarone (5 μM), metoprolol (20 μM), propranolol (20 μM), lidocaine (5 μM), verapamil (2.5 μM), ivabradine (3 μM) for NMCMs, NMCFs, and hPSC-CM.
Animal Study:^[2]	Animal Models 1-day-old neonatal mice; 12-week-old adult mice Dosages 10 mg/kg/d Administration s.c.; ALZET Osmotic Pumps

Cell Assay:^[2]

Cell lines
neonatal mice cardiomyocytes (NMCMs)
Concentrations
10, 20, 40, 50, and 100 μM
Incubation Time
6, 12, 18, and 24 h
Method
In vitro, the neonatal mice cardiomyocytes (NMCMs) are treated with different drugs of different concentration, including isoproterenol (0, 1, 2.5, 5, 10, 20, and 50 μM); amiodarone (0, 1, 2.5, 5, 10, 20, and 50 μM); metoprolol (0, 10, 20, 30, 50, 100, 150, and 200 μM); propranolol (0, 10, 20, 40, 50, and 100 μM); lidocaine (0, 1, 2.5, 5, 10, 20, and 50 μM); verapamil(0, 1, 2.5, 5, 10, 20, and 50 μM);ivabradine (0, 1, 2, 3, 5, 10, and 20 μM). And the concentrations of drugs that promote CMs proliferation most significantly are chosen for subsequent experiments, including isoproterenol (10 μM), amiodarone (5 μM), metoprolol (20 μM), propranolol (20 μM), lidocaine (5 μM), verapamil (2.5 μM), ivabradine (3 μM) for NMCMs, NMCFs, and hPSC-CM.

Animal Study:^[2]

Animal Models
1-day-old neonatal mice; 12-week-old adult mice
Dosages
10 mg/kg/d
Administration
s.c.; ALZET Osmotic Pumps

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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