Rubella Virus capsid Antibody [M17H6] Datasheet

Biological Description

Specificity	Rubella Virus capsid Antibody [M17H6] detects exogenous levels of total Rubella Virus capsid protein.
Background	Rubella virus capsid is the sole capsid protein of the rubella virus within the Rubivirus genus and functions as the core structural component that packages the positive-strand RNA genome and organizes virion assembly at intracellular membranes. The protein contains an N-terminal structural domain that forms the capsid protomer with a unique fold not observed in other viral capsids, and a more flexible C-terminal segment that carries basic and membrane-proximal elements important for RNA binding and interaction with the viral envelope glycoproteins. Capsid binds genomic RNA to form ribonucleoprotein cores and associates with the E1–E2 glycoprotein complex at Golgi-derived membranes, where coordinated interactions between its structural domain and the cytoplasmic tails of glycoproteins drive budding and generation of enveloped virions with defined core geometry. The three-dimensional arrangement of capsid protomers within the virion supports formation of an icosahedrally ordered core that stabilizes the RNA, positions it relative to the envelope, and contributes to overall particle integrity during extracellular transmission. Capsid also participates in virus–host communication by engaging host factors that influence translation and innate defense pathways, including interactions with cellular poly(A)-binding protein that modulate host translation and favor viral gene expression. Phosphorylation of defined residues in the capsid regulates RNA-binding capacity and nucleocapsid assembly, adding a layer of post-translational control that links the capsid–RNA interaction to the timing and efficiency of virion formation. During the rubella virus life cycle, the capsid functions together with nonstructural replicase components and envelope proteins to coordinate replication complex activity, nucleocapsid formation, and budding, embedding it at the interface between genome replication and particle morphogenesis. In infected tissues, the capsid contributes to persistence of viral RNA and maintenance of infectious virions, a property that is relevant to the teratogenic potential of the rubella virus and the pathogenesis of congenital rubella syndrome when infection occurs during early pregnancy.

Specificity

Rubella Virus capsid Antibody [M17H6] detects exogenous levels of total Rubella Virus capsid protein.

Background

Rubella virus capsid is the sole capsid protein of the rubella virus within the Rubivirus genus and functions as the core structural component that packages the positive-strand RNA genome and organizes virion assembly at intracellular membranes. The protein contains an N-terminal structural domain that forms the capsid protomer with a unique fold not observed in other viral capsids, and a more flexible C-terminal segment that carries basic and membrane-proximal elements important for RNA binding and interaction with the viral envelope glycoproteins. Capsid binds genomic RNA to form ribonucleoprotein cores and associates with the E1–E2 glycoprotein complex at Golgi-derived membranes, where coordinated interactions between its structural domain and the cytoplasmic tails of glycoproteins drive budding and generation of enveloped virions with defined core geometry. The three-dimensional arrangement of capsid protomers within the virion supports formation of an icosahedrally ordered core that stabilizes the RNA, positions it relative to the envelope, and contributes to overall particle integrity during extracellular transmission. Capsid also participates in virus–host communication by engaging host factors that influence translation and innate defense pathways, including interactions with cellular poly(A)-binding protein that modulate host translation and favor viral gene expression. Phosphorylation of defined residues in the capsid regulates RNA-binding capacity and nucleocapsid assembly, adding a layer of post-translational control that links the capsid–RNA interaction to the timing and efficiency of virion formation. During the rubella virus life cycle, the capsid functions together with nonstructural replicase components and envelope proteins to coordinate replication complex activity, nucleocapsid formation, and budding, embedding it at the interface between genome replication and particle morphogenesis. In infected tissues, the capsid contributes to persistence of viral RNA and maintenance of infectious virions, a property that is relevant to the teratogenic potential of the rubella virus and the pathogenesis of congenital rubella syndrome when infection occurs during early pregnancy.

Usage Information

Application

WB, ELISA

Dilution

WB

1:1000 - 1:5000

Reactivity

Rubella virus

Source

Mouse Monoclonal Antibody

MW

115 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/24282305/
https://pubmed.ncbi.nlm.nih.gov/33627388/

Application Data

WB

Validated by Selleck

Lane 1: recombinant purified rubella capsid protein, Lane 2: irrelevant viral proteins