RSK1 p90 Antibody [K1J12] Datasheet

Biological Description

Specificity	RSK1 p90 Antibody [K1J12] detects endogenous levels of total RSK1 p90 protein.
Background	RSK1 (p90 ribosomal S6 kinase 1), a serine/threonine kinase of the RSK family positioned at the terminus of the Ras/Raf/MEK/ERK cascade, receives extracellular growth factor signals to drive proliferation, survival, and motility across diverse tissues, including brain, muscle, and epithelia. ERK1/2 docks at its C-terminal kinase domain to initiate sequential phosphorylation culminating in PDK1-mediated activation of the N-terminal catalytic site, enabling RSK1 to phosphorylate over 30 substrates spanning transcription factors, cytoskeletal regulators, and feedback inhibitors. Activated RSK1 docks at the nucleus to phosphorylate CREB at Ser133, inducing immediate early genes like c-Fos and Bcl-2 that promote G1/S transition and apoptosis resistance, while cytoplasmic RSK1 inhibits TSC2 to activate mTORC1/S6K1 for protein synthesis and phosphorylates Mkl1 to enhance SRF transcription of actin cytoskeletal genes driving lamellipodia formation. This dual nuclear-cytoplasmic action integrates immediate mitogenic responses with long-term adaptive gene programs, as RSK1 phosphorylates p27kip1 for cytoplasmic retention to unleash cyclin E-CDK2 while feedback phosphorylating Raf-1 at Ser259 to dampen upstream ERK flux. RSK1 sustains synaptic plasticity through CREB-dependent transcription in hippocampal neurons and coordinates muscle hypertrophy via mTORC1 during exercise, making it a precise readout for researchers dissecting ERK pathway fidelity in neuronal cultures or tumor organoids via phospho-S380 antibodies. Constitutive activation via upstream RAS/RAF mutations fuels prostate cancer progression by enhancing AR transcriptional activity through p300/CBP coactivator synergy and chemoresistance via YB-1 stabilization.

Specificity

RSK1 p90 Antibody [K1J12] detects endogenous levels of total RSK1 p90 protein.

Background

RSK1 (p90 ribosomal S6 kinase 1), a serine/threonine kinase of the RSK family positioned at the terminus of the Ras/Raf/MEK/ERK cascade, receives extracellular growth factor signals to drive proliferation, survival, and motility across diverse tissues, including brain, muscle, and epithelia. ERK1/2 docks at its C-terminal kinase domain to initiate sequential phosphorylation culminating in PDK1-mediated activation of the N-terminal catalytic site, enabling RSK1 to phosphorylate over 30 substrates spanning transcription factors, cytoskeletal regulators, and feedback inhibitors. Activated RSK1 docks at the nucleus to phosphorylate CREB at Ser133, inducing immediate early genes like c-Fos and Bcl-2 that promote G1/S transition and apoptosis resistance, while cytoplasmic RSK1 inhibits TSC2 to activate mTORC1/S6K1 for protein synthesis and phosphorylates Mkl1 to enhance SRF transcription of actin cytoskeletal genes driving lamellipodia formation. This dual nuclear-cytoplasmic action integrates immediate mitogenic responses with long-term adaptive gene programs, as RSK1 phosphorylates p27kip1 for cytoplasmic retention to unleash cyclin E-CDK2 while feedback phosphorylating Raf-1 at Ser259 to dampen upstream ERK flux. RSK1 sustains synaptic plasticity through CREB-dependent transcription in hippocampal neurons and coordinates muscle hypertrophy via mTORC1 during exercise, making it a precise readout for researchers dissecting ERK pathway fidelity in neuronal cultures or tumor organoids via phospho-S380 antibodies. Constitutive activation via upstream RAS/RAF mutations fuels prostate cancer progression by enhancing AR transcriptional activity through p300/CBP coactivator synergy and chemoresistance via YB-1 stabilization.

Usage Information

Application

WB, IP, IHC, IF, FCM

Dilution

WB	IP	IHC	IF	FCM
1:1000	1:70	1:100	1:150-1:200	1:100-1:1000

Reactivity

Mouse, Rat, Human

Source

Rabbit Monoclonal Antibody

MW

83 kDa, 82 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/20557427/
https://pubmed.ncbi.nlm.nih.gov/33972681/

Application Data

WB

Validated by Selleck

Lane 1: MCF7, Lane 2: K562, Lane 3: PC-12, Lane 4: Mouse lung