Rotenone

Catalog No.S2348 Batch:S234802

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Technical Data

Formula

C23H22O6
Molecular Weight 394.42 CAS No. 83-79-4
Solubility (25°C)* In vitro DMSO 78 mg/mL (197.75 mM)
Ethanol 1 mg/mL (2.53 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Rotenone is a botanical insecticide that is an inhibitor of mitochondrial electron transport. This compound inhibits NADH/DB oxidoreductase and NADH oxidase with IC50 of 28.8 nM and 5.1 nM, respectively. It induces apoptosis through enhancing mitochondrial reactive oxygen species production.This chemical can be used to induce animal models of Parkinson's Disease.
In vitro

This compound inhibits NADH/DB oxidoreductase and NADH oxidase, and induces apoptosis through enhancing mitochondrial reactive oxygen species production.[1]
In vivo

This mitochondrial toxin, a mitochondrial toxin, induce the up-regulation of alpha-synuclein (alpha-syn) in PD models.[2]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Naïve CD8+ T cells

  • Concentrations

    0.1 µM

  • Incubation Time

    1 day

  • Method

    OCR of CD8+ T cells are analyzed under basal conditions and after sequential injections of 1 μM oligomycin A, 2 μM carbonyl cyanide p-trifluoromethoxyphenylhydrazone, and 0.1 μM rotenone with 1 μM antimycin A. ECAR is analyzed under basal conditions and after sequential injections of 10 mM glucose, 1 μM oligomycin A, and 50 mM 2-deoxyglucose.

References

  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8641941/
  • https://pubmed.ncbi.nlm.nih.gov/34531546/

Selleck's Rotenone Has Been Cited by 30 Publications

Mitochondrial-cytochrome c oxidase II promotes glutaminolysis to sustain tumor cell survival upon glucose deprivation [ Nat Commun, 2025, 16(1):212] PubMed: 39747079
Inhibition of PTP1B Through NADH Dehydrogenase Activity Reduces Acute Pancreatic Acinar Cell Pyroptosis by Inhibiting the ROS/TXNIP/NLRP3 Pathway [ Dig Dis Sci, 2025, 10.1007/s10620-025-09412-1] PubMed: 41051643
Tumor mitochondrial oxidative phosphorylation stimulated by the nuclear receptor RORγ represents an effective therapeutic opportunity in osteosarcoma [ Cell Rep Med, 2024, 5(5):101519] PubMed: 38692271
SPTBN2 suppresses ferroptosis in NSCLC cells by facilitating SLC7A11 membrane trafficking and localization [ Redox Biol, 2024, 70:103039] PubMed: 38241838
Knockdown of hepatic mitochondrial calcium uniporter mitigates MASH and fibrosis in mice [ Cell Biosci, 2024, 14(1):135] PubMed: 39523398
Novel therapeutic strategies targeting bypass pathways and mitochondrial dysfunction to combat resistance to RET inhibitors in NSCLC [ Biochim Biophys Acta Mol Basis Dis, 2024, 1870(6):167249] PubMed: 38768929
Identifying the potential genes in alpha synuclein driving ferroptosis of Parkinson's disease [ Sci Rep, 2023, 13(1):16893] PubMed: 37803093
Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway [ J Ginseng Res, 2023, 47(1):144-154] PubMed: 36644390
Copper induces cell death by targeting lipoylated TCA cycle proteins [ Science, 2022, 375(6586):1254-1261] PubMed: 35298263
Novel roles of RTN4 and CLIMP-63 in regulating mitochondrial structure, bioenergetics and apoptosis [ Cell Death Dis, 2022, 13(5):436] PubMed: 35508606

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.