Rituximab (anti-CD20)

Catalog No.A2009        Batch: A200906

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Technical Data

CAS No. 174722-31-7
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source CHO cells
Storage
(From the date of receipt)
Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 98.89%
Protein concentration 8.65mg/ml
Endotoxin Level <1EU/mg

Biological Activity

Description Rituximab (anti-CD20) is a chimeric anti-CD20 mAb that binds the CD20 antigen on B cells with a binding affinity of 5 nM, MW: 143.86 KD.
Targets
CD20 [1]
In vitro

Anti-CD20 IgG1 antibody (rituximab), induces strong antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) effects on CD20+ Raji cells with the EC50 at 0.0365 nM and 1.5 nM, respectively.[1]

In vivo

A number of in vivo tumor models suggest the anti-tumor activity of rituximab is dependent, at least in part, on complement[2]. Rituximab can deplete B cells for several months and, as such, could represent an effective therapy for B cell-mediated autoimmune diseases. Rituximab is now widely used in onco-haematology and is currently in development in several autoimmune diseases[3].

Protocol (Only for Reference)

Cell Assay:
  • For ADCC experiments, PBMCs were isolated from healthy human donor blood to serve as effector cells and were plated with target Raji cells at E:T ratio of 50:1 in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Target Raji cells were labeled with calcein-AM for detection of cell lysis prior to experimental set up. Following incubation for 4 hours at 37°C in 5% CO2, supernatants from the experiment were analyzed. For CDC experiments, human complement was plated with calcein-AM labeled target Raji cells in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Following incubation for 4 hours at 37°C in 5% CO2, supernatants from the experiment were analyzed.

Animal Study:
  • Objective: To study the mechanisms of follicular lymphoma’s resistance to rituximab
    Animal Models: Female CB17 severe combined immunodeficient(SCID) mice were injected (s.c.) with rituximab-resistant lymphoma cells (derived from a human FL)
    Formulation: --
    Dosages: 10mg/kg, weekly
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/19188155

    Objective: To investigate the therapeutic effect of rituximab in combination with L19-IL2 on B-cell malignancies
    Animal Models: CB17/lcr severe combined mmunodeficiency (SCID) mice were injected (s.c.) Ramos or DoHH-2 lymphoma cells
    Formulation: saline
    Dosages: 200 μg
    Administration: i.v.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/19005180

    Rituximab can apply to immunodeficient mice (eg: SCID mice), lymphoma cell lines and other related assays (Only for Reference)

Selleck's Rituximab (anti-CD20) has been cited by 11 publications

Noninvasive PET imaging of tumor PD-L1 expression with 64Cu-labeled Durvalumab [ Am J Nucl Med Mol Imaging, 2024, 14(1):31-40] PubMed: 38500749
Daratumumab induces cell-mediated cytotoxicity of primary effusion lymphoma and is active against refractory disease [ Oncoimmunology, 2023, 12(1):2163784] PubMed: 36632565
Development and implementation of natural killer cell simultaneous ADCC and direct killing assay [ Heliyon, 2023, 9(12):e22991] PubMed: 38125417
Rheumatoid factor IgM autoantibodies control IgG homeostasis [ Front Immunol, 2022, 13:1016263] PubMed: 36341420
Beta-Lapachone Attenuates BMSC-Mediated Neuroblastoma Malignant Transformation by Inhibiting Gal-3/Gal-3BP/IL6 Axis [ J Pharmacol Exp Ther, 2022, 381(1):12-21] PubMed: 35078863
A versatile platform for the tumor-targeted delivery of immune checkpoint-blocking immunoglobin G [ J Control Release, 2021, 340:243-258] PubMed: 34752799
Study of the antilymphoma activity of pracinostat reveals different sensitivities of DLBCL cells to HDAC inhibitors [ Blood Adv, 2021, 5(10):2467-2480] PubMed: 33999145
Salt-dependent passive adsorption of IgG1κ-type monoclonal antibodies on hydrophobic microparticles [ Biophys Chem, 2021, 275:106609] PubMed: 33975078
Tumor-Targeting Liposomes with Transient Holes Allowing Intact Rituximab Internally [ Biomacromolecules, 2020, 22(2):723-731] PubMed: 33280388
Maternal embryonic leucine zipper kinase is a novel target for diffuse large B cell lymphoma and mantle cell lymphoma. [ Blood Cancer J, 2019, 9(12):87] PubMed: 31740676

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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