RIF1 Antibody [L2M1] Datasheet

Biological Description

Specificity	RIF1 Antibody [L2M1] detects endogenous levels of total RIF1 protein.
Background	RIF1 (replication timing regulatory factor 1) is a conserved chromatin‑associated protein originally defined as a Rap1‑interacting telomere regulator and now recognized as a central coordinator of DNA replication timing and DNA double‑strand break (DSB) repair pathway choice in vertebrate cells. The protein contains an N‑terminal HEAT‑repeat–rich region that supports higher‑order assembly on chromatin and a C‑terminal segment with multiple short linear motifs, including phosphopeptide‑binding regions that recognize defined phosphorylated epitopes in the N‑terminal domain of 53BP1, allowing stable recruitment of RIF1 to DSB‑proximal chromatin in response to DNA damage signaling. At DNA breaks, RIF1 acts as a key 53BP1 effector and favours classical non‑homologous end joining by limiting 5′‑end resection: RIF1 binding at 53BP1‑marked sites suppresses resection machinery, constrains the generation of extended single‑stranded DNA, and supports ligation‑based repair during G1 and early S phase, with consequences for immunoglobulin class switching and genome integrity at programmed and accidental breaks. RIF1 also contributes to homology‑directed repair in specific contexts and promotes survival under replication stress by protecting nascent DNA at stalled forks from nucleolytic degradation and facilitating fork restart, placing this factor at the interface between damage signaling and replication fork management. In the unperturbed cell cycle, RIF1 functions as a master regulator of the replication‑timing program: it binds preferentially to late‑replicating chromatin domains, interacts with protein phosphatase 1 (PP1), and counteracts DDK‑dependent activation of replication origins, thereby controlling when clusters of origins fire during S phase and contributing to the establishment and maintenance of replication‑timing domains across the genome. Regulation of RIF1 involves transcriptional control by pluripotency and signaling factors such as Oct‑4 and Smad pathways in embryonic stem cells, and post‑translational modification by cell cycle kinases including CDK and ATR–CHK1 axis components, which modulate RIF1 chromatin binding and its ability to restrain dormant origin firing during replication stress.

Specificity

RIF1 Antibody [L2M1] detects endogenous levels of total RIF1 protein.

Background

RIF1 (replication timing regulatory factor 1) is a conserved chromatin‑associated protein originally defined as a Rap1‑interacting telomere regulator and now recognized as a central coordinator of DNA replication timing and DNA double‑strand break (DSB) repair pathway choice in vertebrate cells. The protein contains an N‑terminal HEAT‑repeat–rich region that supports higher‑order assembly on chromatin and a C‑terminal segment with multiple short linear motifs, including phosphopeptide‑binding regions that recognize defined phosphorylated epitopes in the N‑terminal domain of 53BP1, allowing stable recruitment of RIF1 to DSB‑proximal chromatin in response to DNA damage signaling. At DNA breaks, RIF1 acts as a key 53BP1 effector and favours classical non‑homologous end joining by limiting 5′‑end resection: RIF1 binding at 53BP1‑marked sites suppresses resection machinery, constrains the generation of extended single‑stranded DNA, and supports ligation‑based repair during G1 and early S phase, with consequences for immunoglobulin class switching and genome integrity at programmed and accidental breaks. RIF1 also contributes to homology‑directed repair in specific contexts and promotes survival under replication stress by protecting nascent DNA at stalled forks from nucleolytic degradation and facilitating fork restart, placing this factor at the interface between damage signaling and replication fork management. In the unperturbed cell cycle, RIF1 functions as a master regulator of the replication‑timing program: it binds preferentially to late‑replicating chromatin domains, interacts with protein phosphatase 1 (PP1), and counteracts DDK‑dependent activation of replication origins, thereby controlling when clusters of origins fire during S phase and contributing to the establishment and maintenance of replication‑timing domains across the genome. Regulation of RIF1 involves transcriptional control by pluripotency and signaling factors such as Oct‑4 and Smad pathways in embryonic stem cells, and post‑translational modification by cell cycle kinases including CDK and ATR–CHK1 axis components, which modulate RIF1 chromatin binding and its ability to restrain dormant origin firing during replication stress.

Usage Information

Application

WB, IP, IF

Dilution

WB	IP	IF
1:1000	1:50	1:1000

Reactivity

Human, Monkey

Source

Rabbit Monoclonal Antibody

MW

274 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/22850674/
https://pubmed.ncbi.nlm.nih.gov/27066066/

RIF1 Antibody [L2M1]

Biological Description

Usage Information

References

Application Data