PX-12

Catalog No.S7947 Batch:S794702

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Technical Data

Formula

C7H12N2S2
Molecular Weight 188.31 CAS No. 141400-58-0
Solubility (25°C)* In vitro DMSO 38 mg/mL (201.79 mM)
Ethanol 38 mg/mL (201.79 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 2.400mg/ml (12.74mM) Taking the 1 mL working solution as an example, add 50 μL of 48 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description PX-12 (DB05448, 1-methyl propyl 2-imidazolyl disulfide) is a potent thioredoxin-1 (Trx-1) inhibitor by irreversibly thioalkylation of Cys73 of Trx-1. Phase 2.
Targets
Trx-1 [1]
In vitro In MCF-7 and HT-29 cells, PX-12 prevents the hypoxia (1% oxygen)-induced increase in HIF-1alpha protein, and decreases HIF-1-trans-activating activity, VEGF formation, and inducible nitric oxide synthase. This compound also inhibits the growth of MCF-7 and HT-29 cells with IC50s of 1.9 μM and 2.9μM, respectively. It also inhibits HIF-1α protein levels through an Nrf2/PMF-1-mediated increase. In A549 cells, this chemical inhibits cell growth via G2/M phase arrest, and Bax-mediated and ROS-dependent apoptosis. In hepatocelluar carcinoma cells, it exerts a synergistic effect with 5-FU to significantly suppress tumorigenicity.
In vivo In mice bearing MCF-7 tumor xenografts, PX-12 (12 mg/kg, i.p.) decreases HIF-1α and VEGF protein levels and microvessel density.

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    MCF-7 and HT-29 cells

  • Concentrations

    ~10 μM

  • Incubation Time

    72 h

  • Method

    Cell growth is measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cells are exposed to a range of concentrations of PX-12 or this compound for 16 h in air or hypoxia (1% oxygen). The cells are then washed with warm drug-free medium and grown in air for the remainder of the 72-h incubation.
Animal Study:[1]
  • Animal Models

    Mice bearing MCF-7 tumor xenografts

  • Dosages

    12 mg/kg

  • Administration

    i.p.

References

  • https://pubmed.ncbi.nlm.nih.gov/12657718/
  • https://pubmed.ncbi.nlm.nih.gov/21069338/
  • https://pubmed.ncbi.nlm.nih.gov/24172913/
  • https://pubmed.ncbi.nlm.nih.gov/26550453/

Selleck's PX-12 Has Been Cited by 21 Publications

Chrysophanol-mediated trx-1 activation attenuates renal fibrosis through inhibition of the JNK/Cx43 signaling pathway [ Renal Failure, September 5, 2024, 2398710]
Chrysophanol-mediated trx-1 activation attenuates renal fibrosis through inhibition of the JNK/Cx43 signaling pathway [ Renal Failure, September 05 2024, 2398551] PubMed: 39238246
Thioredoxin activity confers resistance against oxidative stress in tumor-infiltrating NK cells [ Journal of Clinical Investigation, October 1, 2020, 5508-5522] PubMed: 32673292
SLC7A11 Is a Superior Determinant of APR-246 (Eprenetapopt) Response than TP53 Mutation Status [ Molecular Cancer Therapeutics, October 01 2021, 1858-1867] PubMed: 34315763
Bakuchiol Attenuates Oxidative Stress and Neuron Damage by Regulating Trx1/TXNIP and the Phosphorylation of AMPK After Subarachnoid Hemorrhage in Mice [ Frontiers in Pharmacology, May 15 2020, 712] PubMed: 32499702
FOXO1 Overexpression Attenuates Tubulointerstitial Fibrosis and Apoptosis in Diabetic Kidneys by Ameliorating Oxidative Stress [ Oxidative Medicine and Cellular Longevity, January 09 2019, 7806046]
Hypoxic Reprogramming of ACOX1-Driven HSP90AB1 Crotonylation Stabilizes Thioredoxin to Orchestrate Redox Homeostasis in Oral Squamous Cell Carcinoma [ Research, February 10 2026, 1129] PubMed: 41675575
Nuclear translocation of thioredoxin-1 promotes colorectal cancer development via modulation of the IL-6/STAT3 signaling axis through interaction with STAT3 [ Theranostics, August 28, 2023, 4730-4744] PubMed: 37771783
Integrating Differential Gene Expression Analysis with Perturbagen-Response Signatures May Identify Novel Therapies for Thyroid-Associated Orbitopathy [ Translational Vision Science & Technology, August 25, 2020, 39] PubMed: 32908802
Substrate Stiffness Modulates Fibroblast Extracellular Vesicle Secretion via Mechanotransduction Pathways [ bioRxiv, 2025, 2025.07.29.667440] PubMed: 40766685

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.