Puromycin

Catalog No.E4996 Batch:E499601

Technical Data

Formula

C₂₂H₂₉N₇O₅

Molecular Weight

471.51

CAS No.

53-79-2

Solubility (25°C)*

In vitro

DMSO

94 mg/mL (199.35 mM)

Ethanol

4 mg/mL (8.48 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.700mg/ml (9.97mM)	Taking the 1 mL working solution as an example, add 50 μL of 94 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.450mg/ml (0.95mM)	Taking the 1 mL working solution as an example, add 50 μL of 9 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Puromycin (CL13900, Puromycine) is an antibiotic, analog of the 3' end of aminoacyl-tRNA and an inhibitor of protein synthesis that mimics tyrosyl-tRNA, causing premature termination of translation. It blocks translation by labeling and releasing elongating polypeptide chains from ribosomes and has been widely used in molecular biology research.

Protocol (from reference)

References

https://pubmed.ncbi.nlm.nih.gov/12732187/#&dopt=Abstract
https://pubmed.ncbi.nlm.nih.gov/10666460/#&dopt=Abstract

Selleck's Puromycin has been cited by 24 publications

Alterations in PD-L1 succinylation shape anti-tumor immune responses in melanoma [ Nat Genet, 2025, 57(3):680-693]	PubMed: 40069506
BRD8 Guards the Pluripotent State by Sensing and Maintaining Histone Acetylation [ Adv Sci (Weinh), 2025, 12(5):e2409160]	PubMed: 39656858
ELTD1 inhibits differentiation of hemogenic endothelium progenitors from human embryonic stem cells through the HPIP-Wnt pathway [ Exp Mol Med, 2025, 57(6):1216-1231]	PubMed: 40456828
A novel pathway for stemness propagation and chemoresistance in non-small cell lung cancer via phosphorylated PKM2-loaded small extracellular vesicles [ Theranostics, 2025, 15(8):3439-3461]	PubMed: 40093893
The de novo DNA methyltransferase 3B is a novel epigenetic regulator of MYC in multiple myeloma, representing a promising therapeutic target to counter relapse [ J Exp Clin Cancer Res, 2025, 44(1):125]	PubMed: 40241199
Keratin 6A promotes skin inflammation through JAK1-STAT3 activation in keratinocytes [ J Biomed Sci, 2025, 32(1):47]	PubMed: 40346694
Unveiling the mechanisms of CEBPD-orchestrated TAM polarization through RGS2/PAR2 pathway and its impact on ICB efficacy in clear cell renal cell carcinoma [ J Immunother Cancer, 2025, 13(7)e010898]	PubMed: 40659446
Repurposing MDM2 inhibitor RG7388 for TP53-mutant NSCLC: a p53-independent pyroptotic mechanism via ROS/p-p38/NOXA/caspase-3/GSDME axis [ Cell Death Dis, 2025, 16(1):452]	PubMed: 40523886
N6-methyladenosine in 28S rRNA promotes oncogenic mRNA translation and tyrosine catabolism [ Cell Rep, 2025, 44(1):115139]	PubMed: 39739529
PTOV1 exerts pro-oncogenic role in colorectal cancer by modulating SQSTM1-mediated autophagic degradation of p53 [ J Transl Med, 2025, 23(1):157]	PubMed: 39905441

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.