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Description
| Specificity | PTEN Antibody detects endogenous levels of PTEN. |
|---|---|
| Background | Phosphatase and tensin homolog deleted on chromosome ten (PTEN) is a phosphatase that is frequently altered in cancer. PTEN has phosphatase-dependent and -independent roles, and genetic alterations in PTEN lead to deregulation of protein synthesis, the cell cycle, migration, growth, DNA repair, and survival signaling. PTEN localization, stability, conformation, and phosphatase activity are controlled by an array of protein-protein interactions and post-translational modifications. Thus, PTEN-interacting and -modifying proteins have profound effects on the tumor suppressive functions of PTEN. PTEN is a dual-specificity protein and lipid phosphatase, and its primary cellular substrate is the second messenger phos-phatidylinositol(3,4,5)-trisphosphate(PIP3), which it hydrolyzes to phosphatidylinositol (4,5)-bisphosphate (PIP2). PTEN blocks phosphatidylinositol 3 kinase (PI3K) signaling by inhibiting PIP3-dependent processes such as the membrane recruitment and activation of AKT, thereby inhibiting cell survival, growth, and proliferation. PTEN thus occupies a critical node for the inhibition of oncogenic transformation. It is also essential for embryonic development. |
Usage Information
| Application | WB,ELISA | ||
|---|---|---|---|
| Dilution |
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| Reactivity | Human Mouse Rat | ||
| MW (kDa) | 54kDa | ||
| Source | Rabbit | ||
| Concentration | 1mg/ml | ||
| Storage buffer | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||
| Storage (From the date of receipt) |
Store at –20°C. |
Application Data
WB