Pinocembrin

Catalog No.S3941 Batch:S394101

Technical Data

Formula	C₁₅H₁₂O₄
Molecular Weight	256.25	CAS No.	480-39-7
Solubility (25°C)*	In vitro	DMSO	51 mg/mL (199.02 mM)
		Ethanol	51 mg/mL (199.02 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Pinocembrin (Dihydrochrysin, Galangin flavanone, 5,7-Dihydroxyflavanone) is a major flavonoid molecule incorporated as multifunctional in the pharmaceutical industry. Its vast range of pharmacological activities has been well researched including antimicrobial, anti-inflammatory, antioxidant, and anticancer activities.
In vitro	Pinocembrin (5,7-dihydroxyflavanone) is one of the primary flavonoids isolated from the variety of plants, mainly from Pinus heartwood, Eucalyptus, Populus, Euphorbia, and Sparattosperma leucanthum, in the diverse flora and purified by various chromatographic techniques. Its vast range of pharmacological activities has been well researched including antimicrobial, anti-inflammatory, antioxidant, and anticancer activities^[1]. Pinocembrin has been shown to increase neuronal viability, decrease lactate dehydrogenase release, inhibit the production of NO and ROS, increase glutathione levels, and downregulate the expression of neuronal NO synthase (nNOS) and iNOS in primary cortical neurons subjected to oxygen-glucose deprivation/reoxygenation (OGD/R)^[2].
In vivo	Pinocembrin can be used as neuroprotective against cerebral ischemic injury with a wide therapeutic time window, which may be attributed to its antiexcitotoxic effects^[1]. It has the ability to reduce reactive oxygen species (ROS), protect the BBB, modulate mitochondrial function, and regulate apoptosis. Pinocembrin (10 mg/kg, i.v.) could reduce brain swelling; improve behavioral deficits; and alleviate neuronal apoptosis, edema of astrocytic end-feet, and the deformation of endothelial cells and capillaries. Pinocembrin reduces endoplasmic reticulum (ER) stress and apoptosis by decreasing C/EBP homologous protein (CHOP)/GADD153 and caspase-12 expression via the PERK-elF2α-ATF4 signaling pathway in MCAO rats. LD50 of i.v. pinocembrin in mice is greater than 700 mg/kg^[2].

Protocol (from reference)

Cell Assay:^{^[4]}	Cell lines SH-SY5Y cells Concentrations 4, 8, 20 μM Incubation Time 1 h Method SH-SY5Y cells are maintained in DMEM containing 10% FBS, 100 units/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere containing 5% CO2 at 37℃. Cells are passaged by trypsinization every 3-5 days. Then cells are seeded at 1 × 10⁴ cells per well into a 96-well plate and then incubated for 24 h at 37 °C in a humidified atmosphere containing 5% CO2. The entire medium is then replaced with fresh medium containing 1% FBS, and one of the compounds is pretreated for 1 h, followed by the addition of 2.0 µg/mL tunicamycin or 30 nM staurosporine. Nuclear staining assays are carried out after a further 24 h of incubation. Cell death is assessed.
Animal Study:^{^[3]}	Animal Models male Sprague-Dawley rats Dosages 22.5 or 67.5 mg/kg Administration i.v.

Cell Assay:^{^[4]}

Cell lines
SH-SY5Y cells
Concentrations
4, 8, 20 μM
Incubation Time
1 h
Method
SH-SY5Y cells are maintained in DMEM containing 10% FBS, 100 units/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere containing 5% CO2 at 37℃. Cells are passaged by trypsinization every 3-5 days. Then cells are seeded at 1 × 10⁴ cells per well into a 96-well plate and then incubated for 24 h at 37 °C in a humidified atmosphere containing 5% CO2. The entire medium is then replaced with fresh medium containing 1% FBS, and one of the compounds is pretreated for 1 h, followed by the addition of 2.0 µg/mL tunicamycin or 30 nM staurosporine. Nuclear staining assays are carried out after a further 24 h of incubation. Cell death is assessed.

Animal Study:^{^[3]}

Animal Models
male Sprague-Dawley rats
Dosages
22.5 or 67.5 mg/kg
Administration
i.v.

References

[4] Izuta H, et al. J Agric Food Chem. 2008, 56(19):8944-53.

+ Expand

Selleck's Pinocembrin has been cited by 2 publications

Pinocembrin alleviates chronic morphine-induced analgesic tolerance and hyperalgesia by inhibiting microglial activation [ Neurol Res, 2022, 1-10]	PubMed: 35574904
Pinocembrin ameliorates intermittent hypoxia-induced neuroinflammation through BNIP3-dependent mitophagy in a murine model of sleep apnea [ J Neuroinflammation, 2020, 17(1):337]	PubMed: 33176803

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.