Picrocrocin

Catalog No.E0242 Batch:E024201

Technical Data

Formula	C₁₆H₂₆O₇
Molecular Weight	330.37	CAS No.	138-55-6
Solubility (25°C)*	In vitro	DMSO	66 mg/mL (199.77 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Picrocrocin, the chemical most responsible for the bitter taste of saffron, is isolated from saffron and inhibits proliferation of cancer cells.
In vitro	Picrocrocin exerts anti-tumor activity through apoptosis, cell cycle arrest, and enhancing the ROS levels and decreases the MMP levels, also inhibiting the JAK/STAT5 signalling pathway in the SK-MEL-2 melanoma cells.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines human SKMEL-2 malignant melanoma cells Concentrations 10, 20 and 40 µM Incubation Time 24 h Method SK-MEL-2 cells are seeded in 6-well plates with 1×10<sup>6</sup> cells per well and then they are treated with 0, 10, 20 and 40 µM picrocrocin for 24 h. This is immediately followed by DAPI (4 ,6-diamidino-2-phenylindole) staining. Then, the cell samples are examined and photographed with a fluorescence microscope. To determine the populations of the apoptotic SK-MEL-2 cells, the cells are platted in 6-well plates and subjected to picrocrocin treatment at different doses (0, 10, 20 and 40 μM) for 24 h. Afterwards, the cells are harvested and PBS-washed. The SK-MEL-2 cells are then treated with Annexin V/ FITC and propidium iodide (PI) for 20 min and the apoptotic cell percentage is checked by flow cytometry.

Cell Assay:^{^[1]}

Cell lines
human SKMEL-2 malignant melanoma cells
Concentrations
10, 20 and 40 µM
Incubation Time
24 h
Method
SK-MEL-2 cells are seeded in 6-well plates with 1×10<sup>6</sup> cells per well and then they are treated with 0, 10, 20 and 40 µM picrocrocin for 24 h. This is immediately followed by DAPI (4 ,6-diamidino-2-phenylindole) staining. Then, the cell samples are examined and photographed with a fluorescence microscope. To determine the populations of the apoptotic SK-MEL-2 cells, the cells are platted in 6-well plates and subjected to picrocrocin treatment at different doses (0, 10, 20 and 40 μM) for 24 h. Afterwards, the cells are harvested and PBS-washed. The SK-MEL-2 cells are then treated with Annexin V/ FITC and propidium iodide (PI) for 20 min and the apoptotic cell percentage is checked by flow cytometry.

References

[1] Yu L,et al. J BUON. 2018 Jul-Aug;23(4):1163-1168.

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