Phospho-Tau (Ser 396) Rabbit mAb

Catalog No.: F3592

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Biological Description

Specificity Phospho-Tau (Ser 396) Rabbit mAb recognizes endogenous levels of Tau protein only when phosphorylated at Ser 396.
Background Phospho-Tau (Ser396) refers to the phosphorylation of the microtubule-associated protein Tau at serine 396, a key modification in normal neuronal function and the pathology of neurodegenerative diseases. Tau is an intrinsically disordered protein composed of an N-terminal projection domain, a proline-rich region, microtubule-binding repeats, and a C-terminal tail that contains the Ser396 residue. Under physiological conditions, phosphorylation at Ser396 regulates Tau’s binding affinity to microtubules, helping to modulate their assembly and stability, which is essential for axonal transport and neuronal integrity. In pathological conditions, such as Alzheimer’s disease and other tauopathies, hyperphosphorylation at Ser396, primarily by kinases like GSK-3β and CDK5, reduces Tau’s association with microtubules, leading to cytoskeletal disintegration and promoting the formation of insoluble neurofibrillary tangles. This impairs neuronal function, disrupts synaptic plasticity, and contributes to neurodegeneration. Phosphorylated Tau at Ser396 results in altered mitochondrial dynamics, disrupted cellular signaling, synaptic dysfunction, and activation of glial and inflammatory responses.

Usage Information

Application WB, IP, IHC Dilution
WB IP IHC
1:1000 1:20 1:1000 - 1:4000
Reactivity Human, Mouse, Rat, Cow
Source Rabbit MW 78 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

Application Data