Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) Antibody [G2C4] Datasheet

Biological Description

Specificity	Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) Antibody [G2C4] detects endogenous levels of total LYN, LCK, HCK, and BLK protein only when it is phosphorylated at Y397, Y394, Y411, and Y389 respectively.
Background	Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) represent the activated forms of Src family kinases (SFKs) that transmit signals from immune receptors through autophosphorylation in their kinase activation loops. These kinases share a conserved SH3-SH2-kinase domain architecture where phosphorylation at homologous tyrosine residues, corresponding to Y416 in c-Src, displaces the activation loop from the catalytic cleft, enabling ATP binding and substrate access while relieving autoinhibitory SH2 clamping by C-terminal tyrosine phosphorylation. Receptor ligation triggers trans-autophosphorylation: LCK Y394 activation upon TCR/CD3 clustering recruits ZAP-70 via tandem ITAM motifs, propagating PLCγ-IP3-Ca2+ flux and NFAT nuclear translocation for T cell effector differentiation; LYN Y397 engages BCR/CD79a/b in B cells to phosphorylate SYK and activate PI3K-AKT-mTOR for survival while balancing ITIM-mediated inhibition through CD22/SHP-1 recruitment; HCK Y411 drives FcγR and integrin signaling in myeloid phagocytes via SYK-Vav-Rac cascades that reorganize actin for podosome formation, NADPH oxidase assembly, and cytokine release; BLK Y389 supports pre-BCR checkpoints through BLNK-SYK coupling that enforces light chain selection during B lymphopoiesis. Dual phosphorylation dynamics, activation loop versus C-terminal tail, integrate positive (CD28, CD40L) and negative (CD45, Csk) regulation to calibrate immune synapse maturation and prevent autoimmunity. These phospho-SFKs govern thymic selection, marginal zone B cell positioning, and macrophage polarization with tissue-specific expression patterns reflecting lineage commitment.

Specificity

Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) Antibody [G2C4] detects endogenous levels of total LYN, LCK, HCK, and BLK protein only when it is phosphorylated at Y397, Y394, Y411, and Y389 respectively.

Background

Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) represent the activated forms of Src family kinases (SFKs) that transmit signals from immune receptors through autophosphorylation in their kinase activation loops. These kinases share a conserved SH3-SH2-kinase domain architecture where phosphorylation at homologous tyrosine residues, corresponding to Y416 in c-Src, displaces the activation loop from the catalytic cleft, enabling ATP binding and substrate access while relieving autoinhibitory SH2 clamping by C-terminal tyrosine phosphorylation. Receptor ligation triggers trans-autophosphorylation: LCK Y394 activation upon TCR/CD3 clustering recruits ZAP-70 via tandem ITAM motifs, propagating PLCγ-IP3-Ca2+ flux and NFAT nuclear translocation for T cell effector differentiation; LYN Y397 engages BCR/CD79a/b in B cells to phosphorylate SYK and activate PI3K-AKT-mTOR for survival while balancing ITIM-mediated inhibition through CD22/SHP-1 recruitment; HCK Y411 drives FcγR and integrin signaling in myeloid phagocytes via SYK-Vav-Rac cascades that reorganize actin for podosome formation, NADPH oxidase assembly, and cytokine release; BLK Y389 supports pre-BCR checkpoints through BLNK-SYK coupling that enforces light chain selection during B lymphopoiesis. Dual phosphorylation dynamics, activation loop versus C-terminal tail, integrate positive (CD28, CD40L) and negative (CD45, Csk) regulation to calibrate immune synapse maturation and prevent autoimmunity. These phospho-SFKs govern thymic selection, marginal zone B cell positioning, and macrophage polarization with tissue-specific expression patterns reflecting lineage commitment.

Usage Information

Application

WB, IP, FCM

Dilution

WB	IP	FCM
1:1000	1:100	1:200-1:800

Reactivity

Human, Mouse

Source

Rabbit Monoclonal Antibody

MW

60 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/22805580/
https://pubmed.ncbi.nlm.nih.gov/28096507/

Application Data

WB

Validated by Selleck

Lane 1: K562, Lane 2: K562 (CIP treated)