Pembrolizumab (anti-PD-1)

Catalog No.A2005       

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Technical Data

CAS No. 1374853-91-4
Formulation PBS buffer, pH 7.2
Isotype Human IgG4
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

Biological Activity

Description Pembrolizumab (anti-PD-1) is a potent, highly selective, fully humanized immunoglobulin (Ig) G4-kappa monoclonal antibody against PD-1 with potential immune checkpoint inhibitory and antineoplastic activities. MW : 146.286 KD.
Targets
PD-1 [1]
(Cell-free assay)
In vitro

The level of LDH and apoptosis are significantly higher in those treated with dexamethasone plus PD1 inhibitor pembrolizumab than other groups.[1]
In vivo

Pembrolizumab and dexamethasone synergistically suppress tumor growth of pancreatic cancer in vivo.[1]

Protocol (Only for Reference)

Cell Assay:

  • Cancer cells were incubated with dexamethasone for 48 h followed by co-cultured with PBMCs with effector/target ratio of 10:1 and the addition of PD1 inhibitor (pembrolizumab; 5 μg/mL) for another 48 h. LDH level was detected.
Animal Study:

  • Animal Models: Immune reconstituted NOD/SCID mice model
    Dosages: 5 mg/kg
    Administration: i.v.
    Reference: [1]
     

Selleck's Pembrolizumab (anti-PD-1) has been cited by 49 publications

Regorafenib plus nivolumab in unresectable hepatocellular carcinoma: the phase 2 RENOBATE trial [ Nat Med, 2024, 10.1038/s41591-024-02824-y] PubMed: 38374347
The BCL-2 inhibitor APG-2575 resets tumor-associated macrophages toward the M1 phenotype, promoting a favorable response to anti-PD-1 therapy via NLRP3 activation [ Cell Mol Immunol, 2024, 21(1):60-79] PubMed: 38062129
Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors [ Life Sci Alliance, 2024, 7(3)e202302368] PubMed: 38176728
[ BMC Cancer, 2024, ] PubMed: 38409035
Acquired resistance to CDK4/6 inhibition is associated with dysregulation of multiple pathways including cyclin D-CDK4/6-RB, EGFR/HER, AKT/mTOR and IFN [ Research Square, 2024, 10.21203/rs.3.rs-3782509/v1] PubMed: none
A20 promotes colorectal cancer immune evasion by upregulating STC1 expression to block "eat-me" signal [ Signal Transduct Target Ther, 2023, 8(1):312] PubMed: 37607946
Circulating extracellular vesicles are monitoring biomarkers of anti-PD1 response and enhancer of tumor progression and immunosuppression in metastatic melanoma [ J Exp Clin Cancer Res, 2023, 42(1):251] PubMed: 37759291
Complement receptor C5aR1 blockade reprograms tumor-associated macrophages and synergizes with anti-PD-1 therapy in gastric cancer [ Int J Cancer, 2023, 153(1):224-237] PubMed: 36799619
Complement receptor C5aR1 blockade reprograms tumor-associated macrophages and synergizes with anti-PD-1 therapy in gastric cancer [ Int J Cancer, 2023, 153(1):224-237] PubMed: 36799619
Expression of PD-1/PD-L1 axis in mediastinal lymph nodes and lung tissue of human and experimental lung fibrosis indicates a potential therapeutic target for idiopathic pulmonary fibrosis [ Respir Res, 2023, 24(1):279] PubMed: 37964265

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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