PDGFR α Rabbit mAb

Catalog No.: F3240

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Biological Description

Specificity PDGFR α Rabbit mAb recognizes endogenous levels of total PDGFR α protein.
Background PDGFRα (Platelet-derived growth factor receptor alpha) is a type III receptor tyrosine kinase characterized by five extracellular immunoglobulin (Ig)-like domains, a single transmembrane domain, and an intracellular region containing a split kinase domain with a unique inserted sequence, a juxtamembrane domain, and a variable C-terminal tail. Activated by ligand-induced dimerization and autophosphorylation, PDGFRα forms homo- or heterodimers (e.g., with PDGFRβ) upon binding PDGF ligands, excluding PDGF-D. PDGFRα is predominantly expressed in cranial neural crest (CNC)-derived mesenchyme and is essential for embryonic development, particularly craniofacial and tooth morphogenesis. It regulates cell proliferation, survival, and migration in a paracrine manner, with PDGFRα expressed in odontogenic mesenchyme and its ligand PDGF-A in dental epithelium throughout all stages of tooth development. Loss of PDGFRα function results in craniofacial defects, mid-facial cleft, and embryonic lethality, highlighting its critical role in CNC-derived tissue development.

Usage Information

Application WB, IP, IHC, IF, FCM, ELISA Dilution
WB IP IHC IF FCM
1:1000 1:30 0.26 µg/Ml (Human, Rat) - 1:500(Mouse) 1:500 1:50
Reactivity Human, Mouse, Rat
Source Rabbit MW 122 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

Application Data