Datasheet

Biological Description

Specificity	Pbx3 Rabbit mAb recognizes endogenous levels of total Pbx3 protein.
Background	Pbx3 (Pre-B-cell leukemia homeobox 3) is a homeodomain-containing transcription factor that plays a pivotal role in developmental gene regulation and disease. It contains a conserved homeodomain for sequence-specific DNA binding, particularly to motifs like 5′-ATCAATCAA-3′, and a PBC domain that mediates interactions with transcription factors such as HOX and MEIS. PBX3 functions primarily as a transcriptional activator, directing the expression of genes involved in embryonic development, organogenesis, hematopoiesis, and neuronal differentiation. It forms heterodimeric or multimeric complexes to enhance DNA-binding specificity and fine-tune transcriptional programs critical for cell identity and tissue maturation. PBX3 is also involved in physiological processes such as respiratory regulation and locomotor activity. In cancer, PBX3 is often overexpressed and acts as an oncogene by promoting cell proliferation, inhibiting apoptosis, and repressing tumor suppressors like p53, thereby driving tumor progression and therapy resistance. Dysregulated PBX3 expression is associated with leukemia, glioma, and colorectal cancer, as well as congenital disorders.

Specificity

Pbx3 Rabbit mAb recognizes endogenous levels of total Pbx3 protein.

Background

Pbx3 (Pre-B-cell leukemia homeobox 3) is a homeodomain-containing transcription factor that plays a pivotal role in developmental gene regulation and disease. It contains a conserved homeodomain for sequence-specific DNA binding, particularly to motifs like 5′-ATCAATCAA-3′, and a PBC domain that mediates interactions with transcription factors such as HOX and MEIS. PBX3 functions primarily as a transcriptional activator, directing the expression of genes involved in embryonic development, organogenesis, hematopoiesis, and neuronal differentiation. It forms heterodimeric or multimeric complexes to enhance DNA-binding specificity and fine-tune transcriptional programs critical for cell identity and tissue maturation. PBX3 is also involved in physiological processes such as respiratory regulation and locomotor activity. In cancer, PBX3 is often overexpressed and acts as an oncogene by promoting cell proliferation, inhibiting apoptosis, and repressing tumor suppressors like p53, thereby driving tumor progression and therapy resistance. Dysregulated PBX3 expression is associated with leukemia, glioma, and colorectal cancer, as well as congenital disorders.

Usage Information

Application

WB

Dilution

WB

1:1000 - 1:10000

Reactivity

Human, Mouse, Rat

Source

Rabbit

MW

47 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

Pbx3 Rabbit mAb

Biological Description

Usage Information

References

Application Data