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Biological Description
| Specificity | Oct4 Rabbit mAb recognizes endogenous levels of total Oct4 protein. |
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| Background | Oct4 (octamer-binding transcription factor 4), encoded by the highly conserved POU5F1 gene, is a key transcription factor critical for maintaining pluripotency in early embryonic development and germ cell lineages. Structurally, Oct4 is a 324-amino acid protein comprising three main domains: an N-terminal transactivation domain, a central POU-specific DNA-binding domain that recognizes the octamer motif (5′-ATTTGCAT-3′), and a C-terminal transactivation domain. Multiple isoforms, including Oct4A, Oct4B, and Oct4B1, differ in their N-terminal regions and biological roles. Oct4 is highly expressed in pluripotent cells such as embryonic stem cells, the inner cell mass of the blastocyst, and primordial germ cells, as well as in immature oocytes. Its expression is tightly regulated—both upregulation and downregulation can disrupt the balance of pluripotency and drive cells toward differentiation. Beyond development, aberrant Oct4 expression has also been observed in several cancers, where it may contribute to tumorigenic potential and cancer stem cell-like traits. |
Usage Information
| Application | WB, IP, IHC, IF, FCM, ChIP | Dilution |
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| Reactivity | Human, Mouse, Rat, Monkey, Sheep, Horse, Cow, Pig | ||||||||||||||
| Source | Rabbit | MW | 38 kDa | ||||||||||||
| Storage Buffer | PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃ | Storage (from the date of receipt) |
-20°C (avoid freeze-thaw cycles), 2 years | ||||||||||||