Datasheet

Biological Description

Specificity	Oct4 Antibody [K18A18] detects endogenous levels of total Oct4 protein.
Background	Oct4 (also known as Oct3/4, OTF3, or NF-A3) is a POU domain-containing transcription factor encoded by the Pou5f1 gene (human chromosome 6, mouse chromosome 17) and is a master regulator of pluripotency. Structurally, it is a ~352 amino acid protein (with human isoforms Oct4A and Oct4B generated by alternative splicing) that contains an N-terminal transactivation domain, a central POU-specific DNA-binding domain, a POU homeodomain, and a C-terminal transactivation domain. Oct4 is expressed in early embryos, embryonic stem (ES) cells, embryonic germ cells, and embryonic carcinoma cells, where its nuclear localization marks pluripotency; expression is later restricted to primordial germ cells and is normally silenced in differentiated somatic tissues. Functionally, Oct4 binds to octamer motifs (ATGCAAAT) in regulatory regions to activate or repress target genes, acting in concert with Sox2 and Nanog to maintain self-renewal, suppress differentiation, and regulate lineage specification. Its dosage-sensitive “rheostat” activity ensures stemness at defined levels, while altered expression drives differentiation toward trophoblast or endodermal lineages. Beyond development, Oct4 is crucial for somatic cell reprogramming into induced pluripotent stem cells and is aberrantly re-expressed in cancer stem cells, where it contributes to tumor initiation, therapy resistance, and relapse.

Specificity

Oct4 Antibody [K18A18] detects endogenous levels of total Oct4 protein.

Background

Oct4 (also known as Oct3/4, OTF3, or NF-A3) is a POU domain-containing transcription factor encoded by the Pou5f1 gene (human chromosome 6, mouse chromosome 17) and is a master regulator of pluripotency. Structurally, it is a ~352 amino acid protein (with human isoforms Oct4A and Oct4B generated by alternative splicing) that contains an N-terminal transactivation domain, a central POU-specific DNA-binding domain, a POU homeodomain, and a C-terminal transactivation domain. Oct4 is expressed in early embryos, embryonic stem (ES) cells, embryonic germ cells, and embryonic carcinoma cells, where its nuclear localization marks pluripotency; expression is later restricted to primordial germ cells and is normally silenced in differentiated somatic tissues. Functionally, Oct4 binds to octamer motifs (ATGCAAAT) in regulatory regions to activate or repress target genes, acting in concert with Sox2 and Nanog to maintain self-renewal, suppress differentiation, and regulate lineage specification. Its dosage-sensitive “rheostat” activity ensures stemness at defined levels, while altered expression drives differentiation toward trophoblast or endodermal lineages. Beyond development, Oct4 is crucial for somatic cell reprogramming into induced pluripotent stem cells and is aberrantly re-expressed in cancer stem cells, where it contributes to tumor initiation, therapy resistance, and relapse.

Usage Information

Application

WB, IP, IHC, IF, FCM, ChIP

Dilution

WB	IP	IHC	IF	FCM	CHIP
1:10000	1:30	1:250	1:110	1:60	8 µg per 107 cells

Reactivity

Human, Mouse

Source

Rabbit Monoclonal Antibody

MW

38kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/25232507/

Oct4 Antibody [K18A18]

Biological Description

Usage Information

References

Application Data