NSC 405020

Catalog No.S8072 Batch:S807201

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Technical Data

Formula

C12H15Cl2NO
Molecular Weight 260.16 CAS No. 7497-07-6
Solubility (25°C)* In vitro DMSO 52 mg/mL (199.87 mM)
Ethanol 52 mg/mL (199.87 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description NSC 405020 is a noncatalytic inhibitor of MT1-MMP, directly interacts with PEX domain of MT1-MMP, affects PEX homodimerization but not catalytic activity of MT1-MMP.
Targets
MT1-MMP [1]
In vitro NSC 405020 is a noncatalytic inhibitor of MT1-MMP, directly interacts with PEX domain of MT1-MMP. NSC 405020 affects PEX homodimerization but not catalytic activity of MT1-MMP. NSC 405020 (100μM) inhibits migration of cells with high level of MT1-MMP, with a reduction of migration efficiency by about 75%. NSC 405020 does not inhibit migration of cells with low level of MT1-MMP and does not inhibit cell adhesion on collagen.[1]
In vivo NSC 405020 (0.5 mg/kg, intratumoral injection) significantly represses tumor growth. NSC 405020 causes a fibrotic, ΔPEX-like tumor phenotype and increases the level of COL-I. [1]

Protocol (from reference)

Kinase Assay:[1]
  • Migration assays

    Assays are conducted in wells of a 24-well, 8 μm pore size Transwell plate. A 6.5-mm insert membrane is coated with 0.1 mL COL-I (300 μg/mL in MEGM) and then air dried for 16 hours. The collagen coating is rehydrated for 1 hour in 0.2 mL MEGM. The inner chamber contains MEGM-10% FBS as a chemoattractant. The compounds (10–100 μmol/L) or DMSO (0.1%–1%) are added to both inner and outer chambers. Before plating in the outer chamber, cells (5×104) are coincubated for 20 minutes with the compounds or DMSO in MEGM. Cells are allowed to migrate for 16 to 18 hours. The cells remaining on the top surface of the membrane are removed with a cotton swab. The cells on the bottom surface of the membrane are fixed and stained (0.2% crystal violet). The incorporated dye is extracted with 1% SDS and the A570 is measured.
Cell Assay:[1]
  • Cell lines

    184B5-MT and MCF7-β3/MT

  • Concentrations

    ~400 μM

  • Incubation Time

    24 or 30 h

  • Method

    Assays are conducted in wells of a 96-well flat bottom, white wall plates. 184B5-MT and MCF7-β3/MT cells (5×104) are grown for 16 hours in MEGM-10% FBS and DMEM-10% FBS, respectively. 184B5-MT cells are replenished with fresh MEGM (0.1 mL per well) and incubated for an additional 24 hours in the presence of the compounds (100 μM) or vehicle (1% DMSO). MCF7-β3/MT cells are replenished with fresh DMEM-10% FBS (0.1 mL per well) and incubated for an additional 6 hours in the presence of the compounds (400 μM) or vehicle (2% DMSO). The viable cells are counted using a luminescent ATP-Lite assay.
Animal Study:[1]
  • Animal Models

    BALB/c nu/nu mice injected with MCF7-β3/WT and MCF7-β3/ΔPEX cells

  • Dosages

    0.5 mg/kg, 3 times per week

  • Administration

    Intratumoral injection

Selleck's NSC 405020 has been cited by 5 publications

MMP14 expression and collagen remodelling support uterine leiomyosarcoma aggressiveness [ Mol Oncol, 2023, 10.1002/1878-0261.13440] PubMed: 37078535
Lymphocyte access to lymphoma is impaired by high endothelial venule regression [ Cell Rep, 2021, 37(4):109878] PubMed: 34706240
Protease Inhibitor-Dependent Inhibition of Light-Induced Stomatal Opening [ Front Plant Sci, 2021, 12:735328] PubMed: 34567048
Lymphatic endothelium stimulates melanoma metastasis and invasion via MMP14-dependent Notch3 and β1-integrin activation. [ Elife, 2018, 7] PubMed: 29712618
LIFR increases the release of soluble endoglin via the upregulation of MMP14 expression in preeclampsia. [ Reproduction, 2018, 155(3):297-306] PubMed: 29363569

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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