Nigericin

Catalog No.E4788 Batch:E478801

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Technical Data

Formula

C40H68O11
Molecular Weight 724.96 CAS No. 28380-24-7
Solubility (25°C)* In vitro Ethanol 100 mg/mL (137.93 mM)
DMSO Insoluble
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%ethyl alcohol 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 1.250mg/ml (1.72mM) Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clarified ethyl alcohol stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% ethyl alcohol 95% Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 1.250mg/ml (1.72mM) Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clear ethyl alcohol stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Nigericin is an antibiotic derived from Streptomyces hygroscopicus that acts as a K+/H+ ionophore, which causes the efflux of potassium from the cell and, thus, influences the mitochondria membrane potential. It also induces pyroptosis in macrophages by activating NLRP3 inflammasome.

Protocol (from reference)

References

  • https://pubmed.ncbi.nlm.nih.gov/20197279/
  • https://pubmed.ncbi.nlm.nih.gov/37370831/

Selleck's Nigericin Has Been Cited by 15 Publications

Targeting CD177: A Novel Therapeutic Strategy for NLRP3-Associated Autoinflammatory Diseases [ International Journal of Molecular Sciences, March 20, 2026, 2841] PubMed: 41898699
Elevated Methionine Flux Drives Pyroptosis Evasion in Persister Cancer Cells [ Cancer Research, March 2, 2023, 720-734] PubMed: 36480167
Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation [ Oxidative Medicine and Cellular Longevity, January 5, 2022, 1733834] PubMed: 35035656
NRG1 Suppresses NLRP3 Inflammasome Activation and Endothelial-Mesenchymal Transition in Cerebral Ischemia-Reperfusion Injury: Association with the AKT/NF-κB Pathway [ Journal of Inflammation Research, February 27, 2026, 19:584926] PubMed: 41798642
Deacetylation of ATG16L1 is required for LC3-associated lysosomal microautophagy [ Autophagy, August 28, 2025, 2948-2962] PubMed: 40851277
2′,4′-dihydroxychalcone alleviates inflammatory bowel disease by inhibiting NLRP3 inflammasome and modulating gut microbiota [ Frontiers in Immunology, 2026, 1751218] PubMed: nan
2′,4′-dihydroxychalcone alleviates inflammatory bowel disease by inhibiting NLRP3 inflammasome and modulating gut microbiota [ Frontiers in Immunology, 2026, 1534567] PubMed: nan
The role of the IL-9‒NLRP3 axis in insulin resistance and adipose tissue inflammation during diet-induced obesity [ Cell Mol Immunol, 2025, 22(11):1478-1490] PubMed: 40962954
p62 mRNA suppresses NLRP1 expression in cutaneous SCC cells through miR-34a-5p [ Cell Death Dis, 2025, 16(1):465] PubMed: 40593496
Human umbilical cord mesenchymal stem cell-derived exosomes repair IBD by activating the SIRT1-FXR pathway in macrophages [ Stem Cell Research & Therapy, 2025, 233] PubMed: 40346712

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.