Myosin IIb Antibody [F18M11] Datasheet

Biological Description

Specificity	Myosin IIb Antibody [F18M11] detects endogenous levels of total Myosin IIb protein.
Background	Myosin IIb, encoded by MYH10, is a non‑muscle class II myosin heavy chain that functions as a central actomyosin motor in vertebrate cells and contributes to cell shape control, migration, cytokinesis, and specialized neuronal functions through ATP‑dependent interaction with filamentous actin. The heavy chain contains an N‑terminal motor domain with actin‑ and ATP‑binding sites, a neck region that associates with essential and regulatory light chains, and a long coiled‑coil tail that supports bipolar filament assembly and isoform‑specific subcellular localization, forming hexameric complexes that generate contractile force along actin bundles. Myosin IIb localizes to the cell rear, perinuclear region, junctional belts, and dendritic spines, where it cooperates with myosin IIa and IIc to organize cortical and stress‑fiber actin networks, couple protrusion at the leading edge to retraction of the cell body, and maintain tension at adherens junctions and intercalated disc–like structures. Motor activity and filament assembly of myosin IIb are regulated by phosphorylation of its regulatory light chain through Ca²⁺/calmodulin‑dependent myosin light‑chain kinase and Rho‑GTP–dependent ROCK, which link Rho‑family signaling to graded activation of actomyosin contractility and determine the spatial distribution of IIa‑ versus IIb‑rich contractile zones during polarized migration and matrix engagement. In migrating endothelial and fibroblast‑like cells, myosin IIb accumulates preferentially at the trailing edge and central region, where its motor‑dependent forward movement on F‑actin and its ability to form and remodel bipolar filaments align crossed actin bundles into antiparallel arrays that transmit traction forces from the lamellipodium to the cell body and coordinate protrusive activity with whole‑cell translocation. In the nervous system, myosin IIb is enriched in dendritic spines and postsynaptic compartments and regulates actin dynamics during synaptic plasticity; activation downstream of long‑term potentiation–inducing stimulation drives reorganization of spine actin into specialized structures that stabilize potentiated synapses and support memory consolidation, while myosin II motor inhibition disrupts these actin rearrangements and impairs maintenance of plasticity. Myosin IIb function integrates with signaling pathways that control spine remodeling and gene expression, including Ca²⁺‑dependent and Rho‑family effectors, and contributes to the mechanical coupling between glutamatergic receptor activation, actin network remodeling, and long‑lasting changes in synaptic efficacy.

Specificity

Myosin IIb Antibody [F18M11] detects endogenous levels of total Myosin IIb protein.

Background

Myosin IIb, encoded by MYH10, is a non‑muscle class II myosin heavy chain that functions as a central actomyosin motor in vertebrate cells and contributes to cell shape control, migration, cytokinesis, and specialized neuronal functions through ATP‑dependent interaction with filamentous actin. The heavy chain contains an N‑terminal motor domain with actin‑ and ATP‑binding sites, a neck region that associates with essential and regulatory light chains, and a long coiled‑coil tail that supports bipolar filament assembly and isoform‑specific subcellular localization, forming hexameric complexes that generate contractile force along actin bundles. Myosin IIb localizes to the cell rear, perinuclear region, junctional belts, and dendritic spines, where it cooperates with myosin IIa and IIc to organize cortical and stress‑fiber actin networks, couple protrusion at the leading edge to retraction of the cell body, and maintain tension at adherens junctions and intercalated disc–like structures. Motor activity and filament assembly of myosin IIb are regulated by phosphorylation of its regulatory light chain through Ca²⁺/calmodulin‑dependent myosin light‑chain kinase and Rho‑GTP–dependent ROCK, which link Rho‑family signaling to graded activation of actomyosin contractility and determine the spatial distribution of IIa‑ versus IIb‑rich contractile zones during polarized migration and matrix engagement. In migrating endothelial and fibroblast‑like cells, myosin IIb accumulates preferentially at the trailing edge and central region, where its motor‑dependent forward movement on F‑actin and its ability to form and remodel bipolar filaments align crossed actin bundles into antiparallel arrays that transmit traction forces from the lamellipodium to the cell body and coordinate protrusive activity with whole‑cell translocation. In the nervous system, myosin IIb is enriched in dendritic spines and postsynaptic compartments and regulates actin dynamics during synaptic plasticity; activation downstream of long‑term potentiation–inducing stimulation drives reorganization of spine actin into specialized structures that stabilize potentiated synapses and support memory consolidation, while myosin II motor inhibition disrupts these actin rearrangements and impairs maintenance of plasticity. Myosin IIb function integrates with signaling pathways that control spine remodeling and gene expression, including Ca²⁺‑dependent and Rho‑family effectors, and contributes to the mechanical coupling between glutamatergic receptor activation, actin network remodeling, and long‑lasting changes in synaptic efficacy.

Usage Information

Application

WB, IP, IHC, IF, FCM

Dilution

WB	IP	IHC	IF	FCM
1:1000	1:30	1:2000	1:100	1:600

Reactivity

Mouse, Rat, Human

Source

Rabbit Monoclonal Antibody

MW

229 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/20797537/
https://pubmed.ncbi.nlm.nih.gov/14617807/

Myosin IIb Antibody [F18M11]

Biological Description

Usage Information

References

Application Data