Datasheet

Biological Description

Specificity	MYL12B Rabbit mAb recognizes endogenous levels of total MYL12B protein.
Background	MYL12B (Myosin Light Chain 12B), also known as MLC-B or MRLC2, is a regulatory light chain of the myosin II complex and plays a critical role in controlling actomyosin contractility in both smooth muscle and non-muscle cells. As part of the EF-hand calcium-binding protein superfamily, MYL12B contains a conserved structure featuring an N-terminal motor domain that interacts with actin, a central "neck" region with isoleucine-glutamine (IQ) motifs that bind calmodulin and modulate motor activity, and a C-terminal tail important for protein-protein interactions and regulation. MYL12B is phosphorylated by kinases such as myosin light chain kinase (MLCK) and Rho-associated kinase (ROCK), particularly at serine and threonine residues within its regulatory region, which enhances actomyosin contractility and affects processes like cell shape, migration, and division. Unlike MYL12A, which is predominantly expressed in smooth muscle, MYL12B is broadly distributed across multiple tissue types including skeletal muscle and non-muscle cells, reflecting its diverse functional roles. MYL12B also acts as a novel ligand for CD69 and contributes to immune cell recruitment in inflammatory conditions, including asthma and eosinophilic chronic rhinosinusitis, making it not only a key player in cytoskeletal dynamics but also a potential therapeutic target in inflammation-related diseases.

Specificity

MYL12B Rabbit mAb recognizes endogenous levels of total MYL12B protein.

Background

MYL12B (Myosin Light Chain 12B), also known as MLC-B or MRLC2, is a regulatory light chain of the myosin II complex and plays a critical role in controlling actomyosin contractility in both smooth muscle and non-muscle cells. As part of the EF-hand calcium-binding protein superfamily, MYL12B contains a conserved structure featuring an N-terminal motor domain that interacts with actin, a central "neck" region with isoleucine-glutamine (IQ) motifs that bind calmodulin and modulate motor activity, and a C-terminal tail important for protein-protein interactions and regulation. MYL12B is phosphorylated by kinases such as myosin light chain kinase (MLCK) and Rho-associated kinase (ROCK), particularly at serine and threonine residues within its regulatory region, which enhances actomyosin contractility and affects processes like cell shape, migration, and division. Unlike MYL12A, which is predominantly expressed in smooth muscle, MYL12B is broadly distributed across multiple tissue types including skeletal muscle and non-muscle cells, reflecting its diverse functional roles. MYL12B also acts as a novel ligand for CD69 and contributes to immune cell recruitment in inflammatory conditions, including asthma and eosinophilic chronic rhinosinusitis, making it not only a key player in cytoskeletal dynamics but also a potential therapeutic target in inflammation-related diseases.

Usage Information

Application

WB, IHC, IF

Dilution

WB	IHC	IF
1:1000-1:10000	1:100 - 1:250	1:250 - 1:500

Reactivity

Human, Mouse, Rat

Source

Rabbit

MW

20 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

[1] Oya R, et al. J Assoc Res Otolaryngol. 2021 Jul;22(4):425-441.

MYL12B Rabbit mAb

Biological Description

Usage Information

References

Application Data