MTA2 Antibody [P2H17] Datasheet

Biological Description

Specificity	MTA2 Antibody [P2H17] detects endogenous levels of total MTA2 protein.
Background	MTA2 forms part of the MTA family within the NuRD chromatin remodeling and histone deacetylase complex, where it scaffolds interactions essential for transcriptional repression across diverse cellular programs. MTA2 features a zinc finger domain alongside BAH and SANT motifs that facilitate DNA and protein binding, enabling its integration into distinct NuRD variants containing MBD3, Sin3a, and CHD4 for targeted chromatin modification. Through these associations, MTA2 recruits HDAC enzymatic cores to deacetylate histones at promoter regions, compacting chromatin and silencing genes involved in differentiation and hormone response pathways. MTA2 directly engages ERα to suppress estrogen-responsive transcription, altering receptor conformation and cofactor recruitment to shift cells toward resistant phenotypes in hormone-dependent contexts. MTA2 collaborates with transcription factors like Aiolos and Ikaros in B cell progenitors, modulating lineage-specific gene networks by enforcing repressive epigenetic marks on developmental loci. This activity extends to broader lineage commitment, where MTA2-containing NuRD complexes balance progenitor proliferation against maturation cues through cyclic modulation of acetyl marks on key regulatory elements.

Specificity

MTA2 Antibody [P2H17] detects endogenous levels of total MTA2 protein.

Background

MTA2 forms part of the MTA family within the NuRD chromatin remodeling and histone deacetylase complex, where it scaffolds interactions essential for transcriptional repression across diverse cellular programs. MTA2 features a zinc finger domain alongside BAH and SANT motifs that facilitate DNA and protein binding, enabling its integration into distinct NuRD variants containing MBD3, Sin3a, and CHD4 for targeted chromatin modification. Through these associations, MTA2 recruits HDAC enzymatic cores to deacetylate histones at promoter regions, compacting chromatin and silencing genes involved in differentiation and hormone response pathways. MTA2 directly engages ERα to suppress estrogen-responsive transcription, altering receptor conformation and cofactor recruitment to shift cells toward resistant phenotypes in hormone-dependent contexts. MTA2 collaborates with transcription factors like Aiolos and Ikaros in B cell progenitors, modulating lineage-specific gene networks by enforcing repressive epigenetic marks on developmental loci. This activity extends to broader lineage commitment, where MTA2-containing NuRD complexes balance progenitor proliferation against maturation cues through cyclic modulation of acetyl marks on key regulatory elements.

Usage Information

Application

WB, IHC, IF, FCM, ELISA

Dilution

WB	IHC	IF	FCM
1:5000-1:50000	1:500-1:2000	1:1000-1:2000	1:10000

Reactivity

Human, Mouse, Rat

Source

Mouse Monoclonal Antibody

MW

75 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/29326122/
https://pubmed.ncbi.nlm.nih.gov/35804097/

Application Data

WB

Validated by Selleck

Lane 1: SW480, Lane 2: Jurkat, Lane 3: K562, Lane 4: NIH/3T3