ML385

Catalog No.S8790 Batch:S879002

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Technical Data

Formula

C29H25N3O4S
Molecular Weight 511.59 CAS No. 846557-71-9
Solubility (25°C)* In vitro DMSO 50 mg/mL (97.73 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
6%DMSO 40%PEG300 5%Tween80 49%ddH2O
1.5mg/ml Taking the 1 mL working solution as an example, add 60 μL 25 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 490 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description ML385 is a novel and specific NRF2 inhibitor with an IC50 of 1.9 μM. It inhibits the downstream target gene expression of NRF2. NRF2 regulates the activity of several ferroptosis and lipid peroxidation-related proteins.
Targets
Ferroptosis [3] Nrf2 [1]
1.9 μM
In vitro

ML385 shows anti-tumor activity in NSCLC (subcutaneous and orthotopic NSCLC models) both as a single agent and in combination with carboplatin. The PK profile tested in CD-1 mice shows that ML385 has a half-life (t1/2 = 2.82 h) after IP injection (30 mg/kg)[1]. ML385 could alleviate the pancreatic injury[2].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    A549 cells

  • Concentrations

    5 μM

  • Incubation Time

    24, 48 and 72 h

  • Method

    --
Animal Study:

[1]
  • Animal Models

    male CD-1 mice

  • Dosages

    30 mg/kg

  • Administration

    i.p.

Selleck's ML385 has been cited by 102 publications

Inhibition of NRF2 enhances the acute myeloid leukemia cell death induced by venetoclax via the ferroptosis pathway [ Cell Death Discov, 2024, 10(1):35] PubMed: 38238299
Hydrophobic Components in Light-Yellow Pulp Sweet Potato (Ipomoea batatas (L.) Lam.) Tubers Suppress LPS-Induced Inflammatory Responses in RAW264.7 Cells via Activation of the Nrf2 Pathway [ Nutrients, 2024, 16(4)563] PubMed: 38398887
Cardioprotective effects of asiaticoside against diabetic cardiomyopathy: Activation of the AMPK/Nrf2 pathway [ J Cell Mol Med, 2024, 28(2):e18055] PubMed: 38113341
Quercetin attenuates cerebral ischemic injury by inhibiting ferroptosis via Nrf2/HO-1 signaling pathway [ Eur J Pharmacol, 2024, 963:176264] PubMed: 38123006
FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice [ Nat Commun, 2023, 14(1):6107] PubMed: 37777507
FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice [ Nat Commun, 2023, 14(1):6107] PubMed: 37777507
Boosting NAD preferentially blunts Th17 inflammation via arginine biosynthesis and redox control in healthy and psoriasis subjects [ Cell Rep Med, 2023, S2666-3791(23)00310-5] PubMed: 37586364
Boosting NAD preferentially blunts Th17 inflammation via arginine biosynthesis and redox control in healthy and psoriasis subjects [ Cell Rep Med, 2023, 4(9):101157] PubMed: 37586364
EP1 activation inhibits doxorubicin-cardiomyocyte ferroptosis via Nrf2 [ Redox Biol, 2023, 65:102825] PubMed: 37531930
Targeting NRF2 uncovered an intrinsic susceptibility of acute myeloid leukemia cells to ferroptosis [ Exp Hematol Oncol, 2023, 12(1):47] PubMed: 37198609

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.