ML385

Catalog No.S8790 Batch:S879002

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Technical Data

Formula

C29H25N3O4S
Molecular Weight 511.59 CAS No. 846557-71-9
Solubility (25°C)* In vitro DMSO 50 mg/mL (97.73 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
6%DMSO 40%PEG300 5%Tween80 49%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 1.500mg/ml (2.93mM) Taking the 1 mL working solution as an example, add 60 μL 25 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 490 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description ML385 is a novel and specific NRF2 inhibitor with an IC50 of 1.9 μM. It inhibits the downstream target gene expression of NRF2. NRF2 regulates the activity of several ferroptosis and lipid peroxidation-related proteins.
Targets
Ferroptosis [3] Nrf2 [1]
1.9 μM
In vitro

ML385 shows anti-tumor activity in NSCLC (subcutaneous and orthotopic NSCLC models) both as a single agent and in combination. The PK profile tested in CD-1 mice shows that ML385 has a half-life (t1/2 = 2.82 h) after IP injection (30 mg/kg)[1]. ML385 could alleviate the pancreatic injury[2].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    A549 cells

  • Concentrations

    5 μM

  • Incubation Time

    24, 48 and 72 h

  • Method

    --
Animal Study:

[1]
  • Animal Models

    male CD-1 mice

  • Dosages

    30 mg/kg

  • Administration

    i.p.

References

  • https://pubmed.ncbi.nlm.nih.gov/27552339/
  • https://www.hindawi.com/journals/omcl/2018/7161592/
  • https://pubmed.ncbi.nlm.nih.gov/32907551/

Selleck's ML385 Has Been Cited by 153 Publications

Sodium nitrate protects against metabolic syndrome by sialin-mediated macrophage rebalance [ Signal Transduct Target Ther, 2025, 10(1):323] PubMed: 41034188
Ferroptosis in acute liver Failure: Unraveling the hepcidin-ferroportin axis and therapeutic interventions [ Redox Biol, 2025, 84:103657] PubMed: 40393152
5-HT regulates resistance to aumolertinib by attenuating ferroptosis in lung adenocarcinoma [ EMBO Mol Med, 2025, 10.1038/s44321-025-00293-5] PubMed: 40897859
Growth differentiation factor 15 aggravates sepsis-induced cognitive and memory impairments by promoting microglial inflammatory responses and phagocytosis [ J Neuroinflammation, 2025, 22(1):44] PubMed: 39985040
Preventive hyperbaric oxygen therapy improves acute graft-versus-host disease by activating the Nrf2/HO-1 pathway [ Front Immunol, 2025, 16:1529176] PubMed: 40083556
SAMSN1 causes sepsis immunosuppression by inducing macrophages to express coinhibitory molecules that cause T-cell exhaustion via KEAP1-NRF2 signaling [ Chin Med J (Engl), 2025, 138(13):1607-1620] PubMed: 40293473
Oridonin mitigates bacterial pneumonia by regulating mitochondrial integrity and ferroptosis via targeting KEAP1/NRF2 signaling [ Biochem Pharmacol, 2025, 242(Pt 2):117310] PubMed: 40925465
Hyperoxia-activated Nrf2 regulates ferroptosis in intestinal epithelial cells and intervenes in inflammatory reaction through COX-2/PGE2/EP2 pathway [ Mol Med, 2025, 31(1):1] PubMed: 39754066
Ligustrazine hydrochloride Prevents Ferroptosis by Activating the NRF2 Signaling Pathway in a High-Altitude Cerebral Edema Rat Model [ Int J Mol Sci, 2025, 26(3)1110] PubMed: 39940878
Transcriptional Repression of CCL2 by KCa3.1 K+ Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M2 Macrophages [ Int J Mol Sci, 2025, 26(15)7624] PubMed: 40806751

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.