Mitoxantrone

Catalog No.S1889 Batch:S188903

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Technical Data

Formula

C22H28N4O6
Molecular Weight 444.48 CAS No. 65271-80-9
Solubility (25°C)* In vitro DMSO 88 mg/mL (197.98 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Mitoxantrone is a type II topoisomerase inhibitor with IC50 of 2.0 μM, 0.42 mM for HepG2 and MCF-7/wt cells, respectively.
Targets
Topo II [1]
(Cell-free assay)
In vitro Mitoxantrone induces DNA fragmentation and the proteolytic cleavage of poly(ADP-ribose) polymerase (PARP), a marker of the activation of caspases, in all the patients studied, demonstrating that the cytotoxic effect of mitoxantrone is due to induction of apoptosis. This compound activates NFkappaB and stimulates IkappaBalpha degradation in the promyelocytic leukemia cell line HL60 but not in the variant cells, HL60/MX2 cells, which lack the beta isoform of topoisomerase II and express a truncated alpha isoform that results in an altered subcellular distribution. It inhibits proliferation of activated PBMCs, B lymphocytes, or antigen-specific T-cell lines (TCLs) stimulated on antigen-presenting cells (APCs) in a dose-dependent manner. This chemical induces apoptosis of PBMCs, monocytes and DCs at low concentrations, whereas higher doses causes cell lysis.
In vivo Mitoxantrone transiently decreases the growth rate of HID xenografts in mice but does not affect that of PAC120 xenografts. This compound results in the severity of the cardiac lesions and the nephropathy and the intestinal toxicity in spontaneously hypertensive rats. This chemical and iron(III) form a strong 2:1 complex, in which it may be acting as a tridentate ligand.

Protocol (from reference)

References

  • https://pubmed.ncbi.nlm.nih.gov/9450803/
  • https://pubmed.ncbi.nlm.nih.gov/10940316/
  • https://pubmed.ncbi.nlm.nih.gov/16171875/
  • https://pubmed.ncbi.nlm.nih.gov/12686819/
  • https://pubmed.ncbi.nlm.nih.gov/9299365/

Customer Product Validation

Blockade of USP11 by pharmacological inhibitor Mitoxantrone destabilizes XIAP protein in MDA-MB-231 cells. MDA-MB-231 cells were treated with Mitoxantrone at indicated concentration for 24 h.

Data from [ , , EBioMedicine, 2017, 15:48-61 ]

Selleck's Mitoxantrone Has Been Cited by 15 Publications

Combined treatment of mitoxantrone sensitizes breast cancer cells to rapalogs through blocking eEF-2K-mediated activation of Akt and autophagy [ Cell Death & Disease, November 03, 2020, 948] PubMed: 33144562
Cellular DNA Topoisomerases Are Required for the Synthesis of Hepatitis B Virus Covalently Closed Circular DNA [ Journal of Virology, May 15, 2019, e02230-18] PubMed: 30867306
Oncolytic Newcastle disease virus induces autophagy-dependent immunogenic cell death in lung cancer cells [ American Journal of Cancer Research, August 01, 2018, 1514-1527] PubMed: 30210920
Elacridar Inhibits BCRP Protein Activity in 2D and 3D Cell Culture Models of Ovarian Cancer and Re-Sensitizes Cells to Cytotoxic Drugs [ Int J Mol Sci, 2025, 26(12)5800] PubMed: 40565261
A Robust Marine Collagen Peptide-Agarose 3D Culture System for In Vitro Modeling of Hepatocellular Carcinoma and Anti-Cancer Therapeutic Development [ Mar Drugs, 2025, 23(10)386] PubMed: 41149589
A 3D Perfusable Platform for In Vitro Culture of Patient Derived Xenografts [ Adv Healthc Mater, 2023, 12(14):e2201434] PubMed: 36461624
Establishment and characterization of NCC-MFS2-C1: a novel patient-derived cancer cell line of myxofibrosarcoma [ Hum Cell, 2020, 10.1007/s13577-020-00420-z] PubMed: 32870449
Cellular DNA Topoisomerases Are Required for the Synthesis of Hepatitis B Virus Covalently Closed Circular DNA [ Journal of Virology, 2019, e02230-18] PubMed: 30867306.0
Identification of Host Proteins Required for Hepatitis B Virus Covalently Closed Circular DNA Biosynthesis by a Chemogenetic Approach [ Drexel University, 2019, 10.17918/s5gd-qd64] PubMed: None
Cariprazine, A Dopamine D2/D3 Receptor Partial Agonist, Modulates ABCG2-Mediated Multidrug Resistance in Cancer [ Cancers, 2018, 308] PubMed: 30181510.0

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.