Datasheet

Biological Description

Specificity	MiTF Mouse mAb detects endogenous levels of total MiTF protein.
Background	The microphthalmia-associated transcription factor (MITF) is a key regulator involved in the differentiation and development of melanocytes and retinal pigment epithelium (RPE). It is also essential for pigment cell-specific transcription of genes encoding melanogenesis enzymes. Heterozygous mutations in the MITF gene are linked to auditory–pigmentary syndromes. MITF exists in at least five isoforms—MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M—which differ at their N-terminal regions and exhibit distinct expression patterns. In mice, the Mitf gene plays a critical role in the development and/or survival of various cell lineages, including melanocytes, RPE, mast cells, and osteoclasts. Both mouse Mitf and human MITF proteins possess a basic helix–loop–helix–leucine zipper (bHLH-LZ) domain, enabling DNA binding and dimerization. All isoforms with extended N-terminal sequences share the same C-terminal portion as MITF-M. MITF-A is the predominant isoform in a fetal-derived human RPE cell line and is also present in multiple other cell types. Its N-terminal “Domain A” shares significant amino acid similarity with the N-terminus of TFE3. MITF serves as a master regulator of melanocyte-specific genes and is indispensable for proper melanocyte lineage development. Dysregulation of MITF activity can contribute to disease pathogenesis, notably melanoma, in which MITF functions as an amplified oncogene. For transcriptional activation, MITF recruits the co-activators CREB-binding protein (CBP) and its homolog p300 to gene promoters. The transcriptional potential of its C-terminal region depends on the N-terminal transactivation domain for full functionality.

Specificity

MiTF Mouse mAb detects endogenous levels of total MiTF protein.

Background

The microphthalmia-associated transcription factor (MITF) is a key regulator involved in the differentiation and development of melanocytes and retinal pigment epithelium (RPE). It is also essential for pigment cell-specific transcription of genes encoding melanogenesis enzymes. Heterozygous mutations in the MITF gene are linked to auditory–pigmentary syndromes. MITF exists in at least five isoforms—MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M—which differ at their N-terminal regions and exhibit distinct expression patterns. In mice, the Mitf gene plays a critical role in the development and/or survival of various cell lineages, including melanocytes, RPE, mast cells, and osteoclasts. Both mouse Mitf and human MITF proteins possess a basic helix–loop–helix–leucine zipper (bHLH-LZ) domain, enabling DNA binding and dimerization. All isoforms with extended N-terminal sequences share the same C-terminal portion as MITF-M. MITF-A is the predominant isoform in a fetal-derived human RPE cell line and is also present in multiple other cell types. Its N-terminal “Domain A” shares significant amino acid similarity with the N-terminus of TFE3. MITF serves as a master regulator of melanocyte-specific genes and is indispensable for proper melanocyte lineage development. Dysregulation of MITF activity can contribute to disease pathogenesis, notably melanoma, in which MITF functions as an amplified oncogene. For transcriptional activation, MITF recruits the co-activators CREB-binding protein (CBP) and its homolog p300 to gene promoters. The transcriptional potential of its C-terminal region depends on the N-terminal transactivation domain for full functionality.

Usage Information

Application

WB, IF, FCM

Dilution

WB

1:1000

Reactivity

Human

Source

Mouse

MW

59 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/11764295/
https://www.nature.com/articles/s41598-023-43207-6

MiTF Mouse mAb

Biological Description

Usage Information

References

Application Data