Melanoma Associated Antigen 100+ / 7 kDa Mouse mAb

Catalog No.: F3175

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Biological Description

Specificity Melanoma Associated Antigen 100+ / 7 kDa Mouse mAb detects endogenous levels of total Melanoma Associated Antigen 100+ / 7 kDa protein.
Background Melanoma Associated Antigen 100+ / 7 kDa (MAGE) proteins are tumor-associated antigens, typically restricted to germ cells of the testis in normal adult tissues but aberrantly expressed in various cancers, including melanoma, glioma, lung, bladder, and multiple myeloma. The “100+/7 kDa” designation refers to variants or isoforms with molecular weights around 100 kDa and 7 kDa. Structurally, MAGE proteins share a conserved ~200 amino acid MAGE homology domain (MHD) composed of two winged helix domains forming a deep cleft for peptide or protein binding, flanked by variable N- and C-terminal regions. Functionally, MAGE proteins can act as oncogenic drivers by interacting with RING-type E3 ubiquitin ligases, modulating protein degradation pathways that regulate apoptosis, proliferation, and metabolism; for example, MAGE-A3 promotes tumor survival by stimulating TRIM28-mediated p53 degradation, whereas MAGE-A4 may induce apoptosis via different protein partners. Their tumor-specific expression, structural plasticity, and oncogenic roles make MAGEs both biomarkers and potential therapeutic targets in cancer immunotherapy.

Usage Information

Application IHC, FCM Dilution
IHC
1:5 - 1:20
Reactivity Human
Source Mouse MW
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/26910052/
  • https://pubmed.ncbi.nlm.nih.gov/23946777/

Application Data