Datasheet

Biological Description

Specificity	MCM2 (J16L17) Rabbit mAb detects endogenous levels of total MCM2 (J16L17) protein.
Background	Minichromosome maintenance proteins (MCMs) are key players in the initiation of DNA replication and are essential for preserving genomic stability. Six conserved subunits—MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7—assemble into a hexameric, ring-shaped complex that functions as a DNA helicase to unwind the double-stranded DNA. Disruption in the expression or activation of the MCM2–7 complex can hinder DNA replication, promote genomic instability, and is strongly linked to tumor development and cancer progression. Among them, MCM2 plays a particularly critical role in regulating DNA replication. Its overexpression is observed in various cancer types, and its dysfunction is associated with tumor progression and poor patient outcomes. Due to this, MCM2 is regarded as a highly sensitive biomarker for cancer diagnosis, prognosis, and predicting chemotherapy responsiveness. The MCM2 gene—formerly referred to as BM28 or CDCL1—is located on chromosome 3q21 and consists of 17 exons. Inhibition of MCM2 has demonstrated anti-tumor effects, suggesting it could be a promising therapeutic target. Moreover, because MCM2 deficiency often leads to DNA replication stress, which can enhance anti-tumor immune responses, targeting MCM2 may also serve to boost the efficacy of cancer immunotherapy.

Specificity

MCM2 (J16L17) Rabbit mAb detects endogenous levels of total MCM2 (J16L17) protein.

Background

Minichromosome maintenance proteins (MCMs) are key players in the initiation of DNA replication and are essential for preserving genomic stability. Six conserved subunits—MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7—assemble into a hexameric, ring-shaped complex that functions as a DNA helicase to unwind the double-stranded DNA. Disruption in the expression or activation of the MCM2–7 complex can hinder DNA replication, promote genomic instability, and is strongly linked to tumor development and cancer progression. Among them, MCM2 plays a particularly critical role in regulating DNA replication. Its overexpression is observed in various cancer types, and its dysfunction is associated with tumor progression and poor patient outcomes. Due to this, MCM2 is regarded as a highly sensitive biomarker for cancer diagnosis, prognosis, and predicting chemotherapy responsiveness. The MCM2 gene—formerly referred to as BM28 or CDCL1—is located on chromosome 3q21 and consists of 17 exons. Inhibition of MCM2 has demonstrated anti-tumor effects, suggesting it could be a promising therapeutic target. Moreover, because MCM2 deficiency often leads to DNA replication stress, which can enhance anti-tumor immune responses, targeting MCM2 may also serve to boost the efficacy of cancer immunotherapy.

Usage Information

Application

WB, IHC, IF, FCM

Dilution

WB	IHC	IF
1:1000- 1:10000	1:200	1:1000

Reactivity

Human, Mouse, Rat

Source

Rabbit

MW

102 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/36303105/

MCM2 (J16L17) Rabbit mAb

Biological Description

Usage Information

References

Application Data