MCC950

Catalog No.S8930 Batch:S893002

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Technical Data

Formula

C20H24N2O5S
Molecular Weight 404.48 CAS No. 210826-40-7
Solubility (25°C)* In vitro DMSO 81 mg/mL (200.25 mM)
Ethanol 7 mg/mL (17.3 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description MCC950 (CP-456773, CRID3) is a potent and selective inhibitor of NLRP3 (NOD-like receptor (NLR) family, pyrin domain-containing protein 3) with IC50 of 7.5 nM and 8.1 nM in BMDMs and HMDMs, respectively.
Targets
NLRP3 [1]
(BMDM-based assay)		 NLRP3 [1]
(HMDM-based assay)
7.5 nM 8.1 nM
In vitro

MCC950 specifically inhibits NLPR3 inflammasome activation.[1]
In vivo

Inhibition of NLRP3 with MCC950 ameliorates proteinuria, renal histological lesions and podocyte foot process effacement in lupus-prone mice.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    NZM2328 podocyte cell line

  • Concentrations

    1 µM

  • Incubation Time

    24 h

  • Method

    The NZM2328 podocyte cell line was stimulated with a control serum or a serum from diseased NZM2328 mice or serum from diseased NZM2328 mice depleted of IgG, with or without MCC950 or Mito TEMPO for 24 hours. After this incubation, cells were subjected to flow cytometry and Western blot analyses for NLRP3 inflammasome activation, mitochondrial membrane potential and production of reactive oxygen species (ROS) analysis.

    (Data sourced from selleck products)
Animal Study:

[1]
  • Animal Models

    female NZM2328, a new NZM strain produced by extended backcrosses of NZB/WF1 to NZW mice

  • Dosages

    10mg/kg

  • Administration

    i.p.

    (Data sourced from selleck products)

Selleck's MCC950 has been cited by 128 publications

LL37 complexed to double-stranded RNA induces RIG-I-like receptor signalling and Gasdermin E activation facilitating IL-36γ release from keratinocytes [ Cell Death Dis, 2025, 16(1):198] PubMed: 40121229
A small molecule directly targets NLRP3 to promote inflammasome activation and antitumor immunity [ Cell Death Dis, 2025, 16(1):252] PubMed: 40185713
H3K18 lactylation-mediated SIX1 upregulation contributes to silica-induced epithelial-mesenchymal transition in airway epithelial cells [ Toxicology, 2025, 514:154109] PubMed: 40049282
Progranulin Plays a Protective Role in Pneumococcal Meningitis by Inhibiting Pyroptosis [ Immun Inflamm Dis, 2025, 13(2):e70140] PubMed: 39887961
cGAS/STING/NLRP3 Signaling Pathway-Mediated Pyroptosis in Hypertrophic Cardiomyopathy Radiotherapy [ Front Biosci (Landmark Ed), 2025, 30(3):26084] PubMed: 40152382
Electroacupuncture Inhibits NLRP3-Mediated Microglial Pyroptosis to Ameliorate Chronic Neuropathic Pain in Rats [ J Pain Res, 2025, 18:1115-1129] PubMed: 40070891
Breast cancer-derived CAV1 promotes lung metastasis by regulating integrin α6β4 and the recruitment and polarization of tumor-associated neutrophils [ Int J Biol Sci, 2024, 20(14):5695-5714] PubMed: 39494337
METTL14 contributes to acute lung injury by stabilizing NLRP3 expression in an IGF2BP2-dependent manner [ Cell Death Dis, 2024, 15(1):43] PubMed: 38218935
Nuclear receptor Rev-erbα alleviates intervertebral disc degeneration by recruiting NCoR-HDAC3 co-repressor and inhibiting NLRP3 inflammasome [ Cell Prolif, 2024, e13720.] PubMed: 39045886
Exosomes from Adipose-Derived Mesenchymal Stromal Cells Prevent Medication-Related Osteonecrosis of the Jaw by Inhibiting Macrophage M1 Polarization and Pyroptosis [ Int J Nanomedicine, 2024, 19:12675-12693] PubMed: 39619056

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.