Lanifibranor (IVA-337)

Catalog No.S8770 Batch:S877001

Technical Data

Formula

C₁₉H₁₅ClN₂O₄S₂

Molecular Weight

434.92

CAS No.

927961-18-0

Solubility (25°C)*

In vitro

DMSO

87 mg/mL (200.03 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution

5%DMSO 1 Corn oil

5.0mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Lanifibranor (IVA-337) is a moderately potent and well balanced pan PPAR agonist with EC50 values of 1537 nM, 866 nM and 206 nM for hPPARα, hPPARδ and hPPARγ, respectively.

Targets

PPARγ ^[1]	PPARδ ^[1]	PPARα ^[1]
206 nM(EC50)	866 nM(EC50)	1537 nM(EC50)

In vitro

IVA337 acts as a pan‐PPAR agonist with moderate and balanced activity on the three PPAR isoforms. IVA337 50% effective concentration (EC50) levels for the human PPARs (hPPARs) were 1.63E-06 M for PPARα, 8.49E-07 M for PPARδ, and 2.28E-07 M for PPARγ. IVA337 EC50 levels for the rodent PPARs were 3.78E-07 M for PPARα, 1.55E-06 M for PPARδ, and 2.23E-07 M for PPARγ. IVA337 inhibits PDGF-induced proliferation, stiffness-induced activation, and TGF-β1-induced overexpression of fibrotic genes in hHSCs (human primary hepatic stellate cells)^[2].

In vivo

Following a single oral dose (10 mg/kg in methyl cellulose as vehicle) of IVA-337 in C57Bl6 mice, plasma pharmacokinetic parameters are evaluated. The Cmax, Tmax and AUCinf are 10710 ng/mL, 1 h and 29367 h·(ng/mL), respectively. The anti-diabetic effect of IVA-337 was evaluated in db/db mice, an obese rodent model of type 2 diabetes characterized by severe insulin resistance, hypertriglyceridemia, marked hyperglycemia. Treatment of db/db mouse with IVA-337 for 5 days induces a dose dependent and significant decrease of circulating glucose levels: 40% at 10 mg/kg, and 58% at 30 mg/kg. In the same study abnormal plasma triglycerides levels observed in this disease model were markedly corrected following treatment with IVA-337: 33% at 10 mg/kg, and 45% at 30 mg/kg. IVA-337 has no effect on hematocrit, plasma volume or heart weight. IVA-337 demonstrates excellent anti-hyperglycemic and hypolipidemic efficacy in the db/db mouse model, and a significant anti-fibrotic activity in the mouse CCl4-induced liver fibrosis model^[1]. IVA337 normalizes insulin sensitivity while controlling body weight gain, adiposity index, and serum triglyceride increases; it decreased liver steatosis, inflammation, and ballooning^[2].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines human primary hepatic stellate cell (hHSC) Concentrations 3 μM Incubation Time 7 days Method Human primary HSCs were seeded on plastic six-well plates for 7 days in complete medium with either dimethyl sulfoxide 0.1% or a compound (IVA337, 3 µM; rosiglitazone, 3 µM; fenofibrate, 30 µM). hHSC activation was evaluated with western blot by measuring the expression of α‐smooth muscle actin (α-SMA).
Animal Study:^{^[1]}	Animal Models C57Bl6 mice Dosages 10 mg/kg Administration oral

Cell Assay:^{^[2]}

Cell lines
human primary hepatic stellate cell (hHSC)
Concentrations
3 μM
Incubation Time
7 days
Method
Human primary HSCs were seeded on plastic six-well plates for 7 days in complete medium with either dimethyl sulfoxide 0.1% or a compound (IVA337, 3 µM; rosiglitazone, 3 µM; fenofibrate, 30 µM). hHSC activation was evaluated with western blot by measuring the expression of α‐smooth muscle actin (α-SMA).

Animal Study:^{^[1]}

Animal Models
C57Bl6 mice
Dosages
10 mg/kg
Administration
oral

References

Selleck's Lanifibranor (IVA-337) has been cited by 1 publication

Berberine exerts neuroprotective activities against cerebral ischemia/reperfusion injury through up-regulating PPAR-γ to suppress NF-κB-mediated pyroptosis [ Brain Res Bull, 2021, 177:22-30]	PubMed: 34517069

Berberine exerts neuroprotective activities against cerebral ischemia/reperfusion injury through up-regulating PPAR-γ to suppress NF-κB-mediated pyroptosis [ Brain Res Bull, 2021, 177:22-30]

PubMed: 34517069

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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