KIF3A Rabbit Recombinant mAb Datasheet

Description

Specificity	KIF3A Rabbit Recombinant mAb detects endogenous level of total KIF3A.
Background	Kinesin superfamily proteins (KIFs) comprise several dozen molecular motor proteins. The KIF3 heterotrimer complex is one of the most abundantly and ubiquitously expressed KIFs in mammalian cells. KIF3A is best known for its role in molecule transport along the axoneme of cilia, and loss of KIF3A causes defects in cilium biogenesis. Also, KIF3A forms a complex with APC and participates in the transport of APC, supporting cell migration and polarization. Additionally, KIF3A constrains the activity of the Wnt/β-catenin pathway by suppressing casein kinase 1 (CK1)-dependent DVL phosphorylation, which is a key step in Wnt signalling.

Specificity

KIF3A Rabbit Recombinant mAb detects endogenous level of total KIF3A.

Background

Kinesin superfamily proteins (KIFs) comprise several dozen molecular motor proteins. The KIF3 heterotrimer complex is one of the most abundantly and ubiquitously expressed KIFs in mammalian cells. KIF3A is best known for its role in molecule transport along the axoneme of cilia, and loss of KIF3A causes defects in cilium biogenesis. Also, KIF3A forms a complex with APC and participates in the transport of APC, supporting cell migration and polarization. Additionally, KIF3A constrains the activity of the Wnt/β-catenin pathway by suppressing casein kinase 1 (CK1)-dependent DVL phosphorylation, which is a key step in Wnt signalling.

Usage Information

Application

WB, IHC,ELISA

Dilution

WB	IHC
1:1000	1:50

Reactivity

Human Mouse Rat

MW (kDa)

80kDa

Source

Rabbit

Concentration

1mg/ml

Storage buffer

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

Storage
(From the date of receipt)

Store at –20°C.

References

Application Data

WB

Validated by Selleck

IHC