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Biological Description
| Specificity | Ki67 Antibody [J16J15] detects endogenous levels of total Ki-67 protein. |
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| Background | Ki-67 is a large nuclear and nucleolar protein encoded by the MKI67 gene and is widely used as a marker of cell proliferation. Structurally, it contains an N-terminal forkhead-associated (FHA) domain, a protein phosphatase 1 (PP1) binding region, central tandem repeats, and a C-terminal leucine/arginine-rich chromatin-binding domain, with multiple isoforms generated through alternative splicing. Ki-67 is expressed in all active phases of the cell cycle (G1, S, G2, and M) but is absent in quiescent (G0) cells, making it a reliable proliferation index in cancer diagnostics. Functionally, it plays critical roles in heterochromatin organization during interphase, assembly of the perichromosomal layer (PCL) during mitosis to prevent chromosome aggregation, and regulation of cell cycle progression through interactions with PP1 and other chromatin-associated proteins. |
Usage Information
| Application | WB, IHC, IF, FCM | Dilution |
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| Reactivity | Human, Mouse, Rat | ||||||||||
| Source | Rabbit Monoclonal Antibody | MW | |||||||||
| Storage Buffer | PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3 | Storage (from the date of receipt) |
-20°C (avoid freeze-thaw cycles), 2 years | ||||||||
References
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