KAT7/HBO1/MYST2 Rabbit Recombinant mAb Datasheet

Description

Specificity	KAT7/HBO1/MYST2 Rabbit Recombinant mAb detects endogenous levels of total KAT7/HBO1/MYST2.
Background	The MYST protein family are catalytic subunits of histone acetyltransferase (HAT) complexes. The MYST protein family are catalytic subunits of histone acetyltransferase (HAT) complexes. The MYST2 HAT is a major mediator of both histone H3 (K14, K23) and H4 (K5, K8, K12) acetylation in vivo. MYST2/HBO1 associates in a combinatorial manner with paralogs JADE1-2-3, the human tumour suppressor proteins ING4 and ING5 and EAF6 in a complex responsible for the majority of histone H4 acetylation in higher eukaryotes. Certain roles of MYST2 are independent of histone acetylation and accordingly MYST2 is known to acetylate several substrates other than histones. Acetylation of non-histone substrates can have an effect on their stability, activity, localisation or interaction with other proteins. MYST2 has been shown to associate with and acetylate several pre-replicative complex subunits, namely ORC1, ORC2, MCM2, CDC6 and geminin, and indeed MYST2 has a role not only in replication licensing through the regulation of pre-replicative complex assembly, but possibly also in the activation of origins of replication and in replication fork movement.

Specificity

KAT7/HBO1/MYST2 Rabbit Recombinant mAb detects endogenous levels of total KAT7/HBO1/MYST2.

Background

The MYST protein family are catalytic subunits of histone acetyltransferase (HAT) complexes. The MYST protein family are catalytic subunits of histone acetyltransferase (HAT) complexes. The MYST2 HAT is a major mediator of both histone H3 (K14, K23) and H4 (K5, K8, K12) acetylation in vivo. MYST2/HBO1 associates in a combinatorial manner with paralogs JADE1-2-3, the human tumour suppressor proteins ING4 and ING5 and EAF6 in a complex responsible for the majority of histone H4 acetylation in higher eukaryotes. Certain roles of MYST2 are independent of histone acetylation and accordingly MYST2 is known to acetylate several substrates other than histones. Acetylation of non-histone substrates can have an effect on their stability, activity, localisation or interaction with other proteins. MYST2 has been shown to associate with and acetylate several pre-replicative complex subunits, namely ORC1, ORC2, MCM2, CDC6 and geminin, and indeed MYST2 has a role not only in replication licensing through the regulation of pre-replicative complex assembly, but possibly also in the activation of origins of replication and in replication fork movement.

Usage Information

Application

WB, IF

Dilution

WB	IF
1:1000	1:50

Reactivity

Human Mouse Rat

MW (kDa)

75kDa

Source

Rabbit

Concentration

1mg/ml

Storage buffer

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

Storage
(From the date of receipt)

Store at –20°C.

References

Application Data

WB

Validated by Selleck

IF